Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS | Thermococcus fumicolans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | while optimal activity is obtained with 2 mM, it is almost totally inhibited with 10 mM MgCl2 | Thermococcus fumicolans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
NAD+ | pH 6.5, 55°C | Thermococcus fumicolans | |
1.16 | - |
ATP | pH 6.5, 55°C | Thermococcus fumicolans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the enzyme does not require monovalent cations to be active. 2 mM KCl or up to 5 mM of NaCl increase its activity up to 70%. Higher concentrations of these cations are inhibitory, but the enzyme still retains 50% activity with 100 mM NaCl or 100 mM KCl | Thermococcus fumicolans | |
Mg2+ | strongly dependent on the MgCl2. While optimal activity is obtained with 2 mM, it is almost totally inhibited with 10 mM MgCl2 | Thermococcus fumicolans | |
Na+ | the enzyme does not require monovalent cations to be active. 2 mM KCl or up to 5 mM of NaCl increase its activity up to 70%. Higher concentrations of these cations are inhibitory, but the enzyme still retains 50% activity with 100 mM NaCl or 100 mM KCl | Thermococcus fumicolans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus fumicolans | Q9HH07 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus fumicolans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | (2) | Thermococcus fumicolans | |
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate | (1) | Thermococcus fumicolans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | the enzyme displays nick joining and blunt-end ligation activity using either ATP or NAD+, as a cofactor | Thermococcus fumicolans | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | the enzyme displays nick joining and blunt-end ligation activity using either ATP or NAD+, as a cofactor | Thermococcus fumicolans | AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tfu DNA ligase | - |
Thermococcus fumicolans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermococcus fumicolans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: 50% of maximal activity, 80°C: about 50% of maximal activity | Thermococcus fumicolans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
3 h, stable | Thermococcus fumicolans |
90 | - |
15 min, 50% loss of activity | Thermococcus fumicolans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermococcus fumicolans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
about 80% of maximal activity, pH 7.0: optimum, pH 7.5: about 10% of maximal activity | Thermococcus fumicolans |