6.3.2.B15: 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanine-L-valine ligase

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For detailed information about 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanine-L-valine ligase, go to the full flat file.

Reaction

(2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate

N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-isoleucine

Synonyms

DdaF

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.2 Acid—amino-acid ligases (peptide synthases)

6.3.2.B15 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanine-L-valine ligase

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Results	in table
1	AA Sequence
1	Cloned(Commentary)
1	General Information
6	kcat/KM [mM/s]
5	KM Value [mM]
5	Natural Substrates/ Products (Substrates)
3	Organism
2	pH Optimum
1	Purification (Commentary)
4	Reaction
3	Reference
13	Substrates and Products (Substrate)
3	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
5	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 6.3.2.B15 - 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanine-L-valine ligase

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4.9

(2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate

Pantoea agglomerans

E2JA31

pH 8.0, 22°C

729882

0.19

L-isoleucine

Pantoea agglomerans

E2JA31

pH 8.0, 22°C

729202

0.024

L-leucine

Pantoea agglomerans

E2JA31

pH 8.0, 22°C

729202

0.52

L-valine

Pantoea agglomerans

E2JA31

pH 8.0, 22°C

729202

56.6

Nbeta-(R,R)-trans-epoxysuccinamoyl-(2S)-2,3-diaminopropionate

Pantoea agglomerans

E2JA31

pH 8.0, 22°C

729882

1.4

Nbeta-(S,S)-trans-epoxysuccinamoyl-(2S)-2,3-diaminopropionate

Pantoea agglomerans

E2JA31

pH 8.0, 22°C

729882