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Literature summary for 6.3.2.B15 extracted from

  • Hollenhorst, M.A.; Clardy, J.; Walsh, C.T.
    The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics (2009), Biochemistry, 48, 10467-10472.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.B15

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pantoea agglomerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 L-valine pH 8.0, 22°C Pantoea agglomerans
0.83
-
 L-isoleucine pH 8.0, 22°C Pantoea agglomerans
7.82
-
 L-leucine pH 8.0, 22°C Pantoea agglomerans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-isoleucine Pantoea agglomerans the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-isoleucine
-
 ?
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-leucine Pantoea agglomerans the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-leucine
-
 ?
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-valine Pantoea agglomerans the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-valine
-
 ?
ATP + 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanine + L-valine Pantoea agglomerans the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides ADP + phosphate + 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanyl-L-valine
-
 ?

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans E2JA31
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pantoea agglomerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-isoleucine
-
 Pantoea agglomerans ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-isoleucine
-
 ?
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-isoleucine the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides Pantoea agglomerans ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-isoleucine
-
 ?
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-leucine
-
 Pantoea agglomerans ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-leucine
-
 ?
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-leucine the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides Pantoea agglomerans ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-leucine
-
 ?
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-valine
-
 Pantoea agglomerans ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-valine
-
 ?
ATP + (2E)-4-[[(2S)-2-amino-2-carboxyethyl]amino]-4-oxobut-2-enoate + L-valine the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides Pantoea agglomerans ADP + phosphate + N-[(2S)-3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-2-methylpropanoyl]-L-valine
-
 ?
ATP + 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanine + L-valine
-
 Pantoea agglomerans ADP + phosphate + 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanyl-L-valine
-
 ?
ATP + 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanine + L-valine the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides Pantoea agglomerans ADP + phosphate + 3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanyl-L-valine
-
 ?
additional information no activity with Nbeta-fumaroyl-(2S)-2,3-diaminopropionate Pantoea agglomerans ?
-
 ?

Synonyms

Synonyms Comment Organism
DdaF
-
 Pantoea agglomerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Pantoea agglomerans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.135
-
 L-valine pH 8.0, 22°C Pantoea agglomerans
0.16
-
 L-isoleucine pH 8.0, 22°C Pantoea agglomerans
0.19
-
 L-leucine pH 8.0, 22°C Pantoea agglomerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pantoea agglomerans

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of fumaramoyl- and epoxysuccinamoyl-dipeptides Pantoea agglomerans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.024
-
 L-leucine pH 8.0, 22°C Pantoea agglomerans
0.19
-
 L-isoleucine pH 8.0, 22°C Pantoea agglomerans
0.52
-
 L-valine pH 8.0, 22°C Pantoea agglomerans