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6.1.1.27: O-phospho-L-serine-tRNA ligase

This is an abbreviated version!
For detailed information about O-phospho-L-serine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.27

Reaction

ATP
+
O-phospho-L-serine
+
tRNACys
=
AMP
+
diphosphate
+
O-phospho-L-seryl-tRNACys

Synonyms

CysRS, Cysteinyl-tRNA synthetase, More, O-phosphoseryl-tRNA synthetase, phosphoseryl-tRNA synthetase, phosphoseryl-tRNA synthetases, SepRS, sepS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.27 O-phospho-L-serine-tRNA ligase

Cloned

Cloned on EC 6.1.1.27 - O-phospho-L-serine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli B834(DE3) cells
expressed in Escherichia coli Rosetta 2(DE3) cells
-
expression in Escherichia coli
-
expression of His-tagged enzyme in Escherichia coli
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli
-
overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), overexpression of SeMet-labeled SepRS in Escherichia coli strain B834(DE3)