5.5.1.7: Chloromuconate cycloisomerase

This is an abbreviated version!
For detailed information about Chloromuconate cycloisomerase, go to the full flat file.

Reaction

Synonyms

2-chloro-5-oxo-2,5-dihydrofuran-2-acetate lyase (decyclizing), 2-chloromuconate cycloisomerase, 2CMA-cycloisomerase, CMC, CMCI, Isomerase, chloromuconate cyclo-, MLE II, Muconate cycloisomerase II, Rho-2-CMCl, tfdD

ECTree

     5 Isomerases

         5.5 Intramolecular lyases

             5.5.1 Intramolecular lyases (only sub-subclass identified to date)

                5.5.1.7 Chloromuconate cycloisomerase

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Results	in table
1119	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
3	Crystallization (Commentary)
4	kcat/KM [mM/s]
27	KM Value [mM]
3	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
116	Organism
17	Pathway
28	PDB ID
4	pH Optimum
2	pH Range
6	Purification (Commentary)
3	Reaction
1	Reaction Type
31	Reference
10	Source Tissue
5	Specific Activity [micromol/min/mg]
2	Storage Stability
75	Substrates and Products (Substrate)
6	Subunits
11	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
20	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 5.5.1.7 - Chloromuconate cycloisomerase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

(+)-2-Chloromuconolactone

trans-4-Carboxymethylenebut-2-en-4-olide + Cl-

2 entries

(+)-5-Chloromuconolactone

trans-4-Carboxymethylenebut-2-en-4-olide + Cl-

3 entries

2,4-dichloro-cis,cis-muconate

2-chlorodienelactone

Cupriavidus necator

-

-

747612

-

-

?

2,4-dichloro-cis,cis-muconate

?

4 entries

2,5-Dichloro-cis,cis-muconate

?

Xanthobacter flavus

-

-

3757

-

-

?

2-chloro-3-methylmuconate

5-chloro-4-methylmuconolactone + 2-chloro-3-methylmuconolactone

2 entries

2-chloro-4-methylmuconate

5-chloro-3-methylmuconolactone + 2-chloro-4-methylmuconolactone

2 entries

2-Chloro-cis,cis-muconate

4-Carboxymethyl-2-chloro-but-2-en-4-olide

7 entries

2-Chloro-cis,cis-muconate

5-Chloromuconolactone

3 entries

2-Methyl-cis,cis-muconate

?

5 entries

3-Bromo-cis,cis-muconate

5-bromomuconolactone

2 entries

3-chloro-2-methylmuconate

5-methyl-cis-dienelactone + 2-chloro-3-methylmuconolactone

2 entries

3-chloro-2-methylmuconolactone + 3-chloro-2-methylmuconolactone

?

2 entries

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

10 entries

3-Fluoro-cis,cis-muconate

?

Rhodococcus erythropolis

-

-

3656

-

-

?

3-Methyl-cis,cis-muconate

?

4 entries

4-chloro-2-methylmuconate

2-methyl-cis-dienelactone + 3-chloro-5-methylmuconolactone

2 entries

cis,cis-Muconate

?

7 entries

additional information

?

-

15 entries

(+)-2-Chloromuconolactone

trans-4-Carboxymethylenebut-2-en-4-olide + Cl-

Cupriavidus necator

-

-

3760

-

3760

?

(+)-2-Chloromuconolactone

trans-4-Carboxymethylenebut-2-en-4-olide + Cl-

Escherichia coli

-

-

3760

-

3760

?

(+)-5-Chloromuconolactone

trans-4-Carboxymethylenebut-2-en-4-olide + Cl-

Cupriavidus necator

-

-

3760

-

3760

?

(+)-5-Chloromuconolactone

trans-4-Carboxymethylenebut-2-en-4-olide + Cl-

Escherichia coli

-

-

3760

-

3760

?

(+)-5-Chloromuconolactone

trans-4-Carboxymethylenebut-2-en-4-olide + Cl-

Rhodococcus erythropolis

-

elimination of chloride is not catalyzed

3656

-

-

?

2,4-dichloro-cis,cis-muconate

?

Cupriavidus necator

-

-

3760

-

-

?

2,4-dichloro-cis,cis-muconate

?

Escherichia coli

-

-

3760

-

-

?

2,4-dichloro-cis,cis-muconate

?

Rhodococcus erythropolis

-

-

3656

-

-

?

2,4-dichloro-cis,cis-muconate

?

Xanthobacter flavus

-

-

3757

-

-

?

2-chloro-3-methylmuconate

5-chloro-4-methylmuconolactone + 2-chloro-3-methylmuconolactone

Ralstonia sp.

-

-

662030

-

-

?

2-chloro-3-methylmuconate

5-chloro-4-methylmuconolactone + 2-chloro-3-methylmuconolactone

Ralstonia sp. PS12

-

-

662030

-

-

?

2-chloro-4-methylmuconate

5-chloro-3-methylmuconolactone + 2-chloro-4-methylmuconolactone

Ralstonia sp.

-

-

662030

-

-

?

2-chloro-4-methylmuconate

5-chloro-3-methylmuconolactone + 2-chloro-4-methylmuconolactone

Ralstonia sp. PS12

-

-

662030

-

-

?

2-Chloro-cis,cis-muconate

4-Carboxymethyl-2-chloro-but-2-en-4-olide

Cupriavidus necator

-

-

3760

trans-4-carboxymethylenebut-2-en-4-olide is produced after dehalogenation

3760

?

2-Chloro-cis,cis-muconate

4-Carboxymethyl-2-chloro-but-2-en-4-olide

Escherichia coli

-

-

3760

trans-4-carboxymethylenebut-2-en-4-olide is produced after dehalogenation

3760

?

2-Chloro-cis,cis-muconate

4-Carboxymethyl-2-chloro-but-2-en-4-olide

Pseudomonas sp.

-

-

3748

-

3748

?

2-Chloro-cis,cis-muconate

4-Carboxymethyl-2-chloro-but-2-en-4-olide

Pseudomonas sp.

-

65% of the activity relative to 2-methyl-cis,cis-muconate

3634

trans-4-carboxymethylenebut-2-en-4-olide is produced after dehalogenation

3634

?

2-Chloro-cis,cis-muconate

4-Carboxymethyl-2-chloro-but-2-en-4-olide

Pseudomonas sp. B13

-

-

3748

-

3748

?

2-Chloro-cis,cis-muconate

4-Carboxymethyl-2-chloro-but-2-en-4-olide

Rhodococcus opacus

Q8G9L1

-

729312

-

-

?

2-Chloro-cis,cis-muconate

4-Carboxymethyl-2-chloro-but-2-en-4-olide

Xanthobacter flavus

-

-

3757

-

-

?

2-Chloro-cis,cis-muconate

5-Chloromuconolactone

Rhodococcus erythropolis

-

r

3656

the enzyme is unable to catalyze dehalogenation

3656

?

2-Chloro-cis,cis-muconate

5-Chloromuconolactone

Rhodococcus opacus

-

-

650268

-

650268

?

2-Chloro-cis,cis-muconate

5-Chloromuconolactone

Rhodococcus opacus 1CP

-

-

650268

-

650268

?

2-Methyl-cis,cis-muconate

?

Delftia acidovorans

-

-

3647

-

-

?

2-Methyl-cis,cis-muconate

?

Delftia acidovorans CA28

-

-

3647

-

-

?

2-Methyl-cis,cis-muconate

?

Pseudomonas sp.

-

most effective substrate

3634

-

-

?

2-Methyl-cis,cis-muconate

?

Rhodococcus erythropolis

-

-

3656

-

-

?

2-Methyl-cis,cis-muconate

?

Xanthobacter flavus

-

-

3757

-

-

?

3-Bromo-cis,cis-muconate

5-bromomuconolactone

Pseudomonas sp.

-

-

3646

-

3646

?

3-Bromo-cis,cis-muconate

5-bromomuconolactone

Pseudomonas sp. B13

-

-

3646

-

3646

?

3-chloro-2-methylmuconate

5-methyl-cis-dienelactone + 2-chloro-3-methylmuconolactone

Ralstonia sp.

-

-

662030

-

-

?

3-chloro-2-methylmuconate

5-methyl-cis-dienelactone + 2-chloro-3-methylmuconolactone

Ralstonia sp. PS12

-

-

662030

-

-

?

3-chloro-2-methylmuconolactone + 3-chloro-2-methylmuconolactone

?

Ralstonia sp.

-

-

662030

-

-

?

3-chloro-2-methylmuconolactone + 3-chloro-2-methylmuconolactone

?

Ralstonia sp. PS12

-

-

662030

-

-

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Cupriavidus necator

-

-

3760

-

-

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Delftia acidovorans

-

about 90% of the activity compared to 2-methyl-cis,cis-muconate

3647

-

-

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Delftia acidovorans CA28

-

about 90% of the activity compared to 2-methyl-cis,cis-muconate

3647

-

-

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Escherichia coli

-

-

3760

-

-

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Pseudomonas sp.

-

-

3646

corresponding (E)-dienenlactone

3646

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Pseudomonas sp.

-

73% of the activity relative to 2-methyl-cis,cis-muconate

3634

-

3634

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Pseudomonas sp. B13

-

-

3646

corresponding (E)-dienenlactone

3646

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Rhodococcus erythropolis

-

-

3656

-

-

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Rhodococcus opacus

Q8G9L1

-

729312

-

-

?

3-Chloro-cis,cis-muconate

cis-4-Carboxymethylenebut-2-en-4-olide

Xanthobacter flavus

-

-

3757

-

-

?

3-Methyl-cis,cis-muconate

?

Pseudomonas sp.

-

84% of the activity relative to 2-methyl-cis,cis-muconate

3634

-

-

?

3-Methyl-cis,cis-muconate

?

Rhodococcus erythropolis

-

-

3656

-

-

?

3-Methyl-cis,cis-muconate

?

Rhodococcus opacus

Q8G9L1

-

729312

-

-

?

3-Methyl-cis,cis-muconate

?

Xanthobacter flavus

-

-

3757

-

-

?

4-chloro-2-methylmuconate

2-methyl-cis-dienelactone + 3-chloro-5-methylmuconolactone

Ralstonia sp.

-

-

662030

-

-

?

4-chloro-2-methylmuconate

2-methyl-cis-dienelactone + 3-chloro-5-methylmuconolactone

Ralstonia sp. PS12

-

-

662030

-

-

?

cis,cis-Muconate

?

Cupriavidus necator

-

-

3760

-

-

?

cis,cis-Muconate

?

Delftia acidovorans

-

about 30% of the activity compared to 2-methyl-cis,cis-muconate

3647

-

-

?

cis,cis-Muconate

?

Delftia acidovorans CA28

-

about 30% of the activity compared to 2-methyl-cis,cis-muconate

3647

-

-

?

cis,cis-Muconate

?

Pseudomonas sp.

-

36% of the activity relative to 2-methyl-cis,cis-muconate

3634

-

-

?

cis,cis-Muconate

?

Rhodococcus erythropolis

-

-

3656

-

-

?

cis,cis-Muconate

?

Rhodococcus opacus

Q8G9L1

-

729312

-

-

?

cis,cis-Muconate

?

Xanthobacter flavus

-

-

3757

-

-

?

additional information

?

-

Alcaligenes sp.

-

completely inactive with 2-fluoro-cis,cis-muconate

3753

-

-

?

additional information

?

-

Alcaligenes sp. A7-2

-

completely inactive with 2-fluoro-cis,cis-muconate

3753

-

-

?

additional information

?

-

Cupriavidus necator

-

induced at low levels in strain RD330 by 3-chlorobenzoate and its metabolites

3751

-

-

?

additional information

?

-

Cupriavidus necator

-

enzyme is involved in the 2,4-dichlorophenoxyacetate degradation pathway

3756

-

-

?

additional information

?

-

Cupriavidus necator RD330

-

induced at low levels in strain RD330 by 3-chlorobenzoate and its metabolites

3751

-

-

?

additional information

?

-

Pseudomonas sp.

-

completely inactive with 2-fluoro-cis,cis-muconate

3753

-

-

?

additional information

?

-

Pseudomonas sp.

-

the enzyme is involved in the degradation of 3,5-dichlorocatechol

3754

-

-

?

additional information

?

-

Pseudomonas sp. B13

-

the enzyme is involved in the degradation of 3,5-dichlorocatechol

3754

-

-

?

additional information

?

-

Pseudomonas sp. B13

-

completely inactive with 2-fluoro-cis,cis-muconate

3753

-

-

?

additional information

?

-

Rhodococcus erythropolis

-

completely inactive with 2-fluoro-cis,cis-muconate

3656

-

-

?

additional information

?

-

Rhodococcus opacus

-

no activity with cis,cis-muconate, 3-chloromuconate, 3-methylmuconate and 2,4-dichloromuconate

650268

-

650268

?

additional information

?

-

Rhodococcus opacus

-

key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway

650268

-

650268

?

additional information

?

-

Rhodococcus opacus 1CP

-

no activity with cis,cis-muconate, 3-chloromuconate, 3-methylmuconate and 2,4-dichloromuconate

650268

-

650268

?

additional information

?

-

Rhodococcus opacus 1CP

-

key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway

650268

-

650268

?

additional information

?

-

Xanthobacter flavus

-

rate-limiting step in the conversion of dichloromuconate to chloromaleylacetate

3757

-

-

?