Crystallization (Comment) | Organism |
---|---|
the crystallographic structure of the homooctameric Rho-2-CMCI is solved at 2.5 A resolution by molecular replacement using the coordinates of the structure of chloromuconate cycloisomerase from Pseudomonas putida PRS2000 | Rhodococcus opacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0487 | - |
3-methyl-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
0.0559 | - |
2-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
0.0813 | - |
cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
1.24 | - |
3-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | Q8G9L1 | - |
- |
Purification (Comment) | Organism |
---|---|
anion-exchange chromatography on DEAE-Toyopearl, hydrophobic chromatography on Phenyl-sepharose with linear gradient (NH4)2SO4, anion-exchange Q3 chromatography and hydrophobic chromatography | Rhodococcus opacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Chloro-cis,cis-muconate | - |
Rhodococcus opacus | 4-Carboxymethyl-2-chloro-but-2-en-4-olide | - |
? | |
3-Chloro-cis,cis-muconate | - |
Rhodococcus opacus | cis-4-Carboxymethylenebut-2-en-4-olide | - |
? | |
3-Methyl-cis,cis-muconate | - |
Rhodococcus opacus | ? | - |
? | |
cis,cis-Muconate | - |
Rhodococcus opacus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | - |
Rhodococcus opacus |
Synonyms | Comment | Organism |
---|---|---|
2-chloromuconate cycloisomerase | - |
Rhodococcus opacus |
Rho-2-CMCl | - |
Rhodococcus opacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
3-methyl-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
3.6 | - |
2-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
8.8 | - |
3-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
24.66 | - |
cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.66 | - |
3-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
65 | - |
2-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
65 | - |
3-methyl-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
303 | - |
cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus |