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Literature summary for 5.5.1.7 extracted from

  • Solyanikova, I.P.; Maltseva, O.V.; Vollmer, M.D.; Golovleva, L.A.; Schlömann, M.
    Characterization of muconate and chloromuconate cycloisomerase from Rhodococcus erythropolis 1CP: indications for functionally convergent evolution among bacterial cycloisomerases (1995), J. Bacteriol., 177, 2821-2826.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.5.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 2-methyl-cis,cis-muconate
-
 Rhodococcus erythropolis
0.052
-
 3-fluoro-cis,cis-muconate
-
 Rhodococcus erythropolis
0.066
-
 3-methyl-cis,cis-muconate
-
 Rhodococcus erythropolis
0.119
-
 2,4-dichloro-cis,cis-muconate
-
 Rhodococcus erythropolis
0.173
-
 3-chloro-cis,cis-muconate
-
 Rhodococcus erythropolis
0.312
-
 2-chloro-cis,cis-muconate
-
 Rhodococcus erythropolis
6.997
-
 cis,cis-muconate
-
 Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000 250000 gel filtration, nondenaturing electrophoresis Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
 1CP
-
Rhodococcus erythropolis 1CP
-
 1CP
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus erythropolis

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-chlorophenol-grown cell
-
 Rhodococcus erythropolis
-
culture condition:benzoate-grown cell
-
 Rhodococcus erythropolis
-

Storage Stability

Storage Stability Organism
4°C, enzyme concentration 0.45 mg/ml, 50 mM Tris-HCl buffer, pH 7.5, 2 mM MnSO4, 0.2 M NaCl, 50% loss of activity after 3.5 months Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-5-Chloromuconolactone elimination of chloride is not catalyzed Rhodococcus erythropolis trans-4-Carboxymethylenebut-2-en-4-olide + Cl-
-
 ?
2,4-dichloro-cis,cis-muconate
-
 Rhodococcus erythropolis ?
-
 ?
2-Chloro-cis,cis-muconate r Rhodococcus erythropolis 5-Chloromuconolactone the enzyme is unable to catalyze dehalogenation ?
2-Methyl-cis,cis-muconate
-
 Rhodococcus erythropolis ?
-
 ?
3-Chloro-cis,cis-muconate
-
 Rhodococcus erythropolis cis-4-Carboxymethylenebut-2-en-4-olide
-
 ?
3-Fluoro-cis,cis-muconate
-
 Rhodococcus erythropolis ?
-
 ?
3-Methyl-cis,cis-muconate
-
 Rhodococcus erythropolis ?
-
 ?
cis,cis-Muconate
-
 Rhodococcus erythropolis ?
-
 ?
additional information completely inactive with 2-fluoro-cis,cis-muconate Rhodococcus erythropolis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000-42000, SDS-PAGE Rhodococcus erythropolis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0567
-
 2-methyl-cis,cis-muconate
-
 Rhodococcus erythropolis
0.8
-
 3-methyl-cis,cis-muconate
-
 Rhodococcus erythropolis
2.2
-
 2-chloro-cis,cis-muconate
-
 Rhodococcus erythropolis
4.33
-
 cis,cis-muconate
-
 Rhodococcus erythropolis
6.07
-
 3-fluoro-cis,cis-muconate
-
 Rhodococcus erythropolis
22.3
-
 2,4-dichloro-cis,cis-muconate
-
 Rhodococcus erythropolis
60.7
-
 3-chloro-cis,cis-muconate
-
 Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rhodococcus erythropolis

pH Range

pH Minimum pH Maximum Comment Organism
7.2 8.2 4 C, 36 h, 40 mM Tris-HCl, MOPS or HEPES buffer, enzyme concentration 0.03 mg/ml Rhodococcus erythropolis