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5.4.99.40: alpha-amyrin synthase

This is an abbreviated version!
For detailed information about alpha-amyrin synthase, go to the full flat file.

Word Map on EC 5.4.99.40

Reaction

(3S)-2,3-epoxy-2,3-dihydrosqualene
=
alpha-amyrin

Synonyms

2,3-oxidosqualene alpha-amyrin cyclase, alpha amyrin synthase, alpha-amyrin synthase, alpha/beta amyrin synthase, BmOSC, CrAS, MdOSC1, MdOSC3, mixed amyrin synthase, multifunctional OSC, ObAS2, OPSC 1, OSC, OSC1, OSC3, OSCPSM, Oxidosqualene cyclase, oxidosqualene cyclases, PSM, SlTTS1

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.40 alpha-amyrin synthase

Cloned

Cloned on EC 5.4.99.40 - alpha-amyrin synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed by transient expression in Nicotiana benthamiana leaves and by expression in the yeast Pichia methanolica
expressed in Escherichia coli
expressed in Nicotiana benthamiana leaves and in Pichia methanolica
expressed in Saccharomyces cerevisiae mutant GIL77
-
expressed in yeast
-
expression of PSM in ERG7 deficient yeast mutant GIL77
functional expressions of ObAS1 in Saccharomyces cerevisiae leads to the production of beta-amyrin
-
gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. MdOSC1 produces predominantly alpha-amyrin, beta-amyrin to a lesser extent, and small amounts of lupeol
gene OSC3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol