Information on EC 5.4.99.40 - alpha-amyrin synthase

Word Map on EC 5.4.99.40
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.4.99.40
-
RECOMMENDED NAME
GeneOntology No.
alpha-amyrin synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3S)-2,3-epoxy-2,3-dihydrosqualene = alpha-amyrin
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alpha-amyrin biosynthesis
-
-
mangrove triterpenoid biosynthesis
-
-
pentacyclic triterpene biosynthesis
-
-
Sesquiterpenoid and triterpenoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(3S)-2,3-epoxy-2,3-dihydrosqualene mutase (cyclizing, alpha-amyrin-forming)
A multifunctional enzyme which produces both alpha- and beta-amyrin (see EC 5.4.99.39, beta-amyrin synthase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
Royal Gala
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
cultivars MicroTom, M82, and Ailsa Craig
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
functional expressions of ObAS2 in Saccharomyces cerevisiae leads to the production of both alpha-amyrin and beta-amyrin but with a preference for alpha-amyrin
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3S)-2,3-epoxy-2,3-dihydrosqualene
alpha-amyrin
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3S)-2,3-epoxy-2,3-dihydrosqualene
alpha-amyrin
show the reaction diagram
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
weak expression in floral parts
Manually annotated by BRENDA team
high expression
Manually annotated by BRENDA team
weak expression
Manually annotated by BRENDA team
high expression
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
87620
calculated from cDNA
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
thin-layer chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed by transient expression in Nicotiana benthamiana leaves and by expression in the yeast Pichia methanolica
expressed in Escherichia coli
expressed in Saccharomyces cerevisiae mutant GIL77
-
expression of PSM in ERG7 deficient yeast mutant GIL77
functional expressions of ObAS1 in Saccharomyces cerevisiae leads to the production of beta-amyrin
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
high expression in stem and leaves