5.4.99.23: 23S rRNA pseudouridine1911/1915/1917 synthase
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For detailed information about 23S rRNA pseudouridine1911/1915/1917 synthase, go to the full flat file.
Reaction
Synonyms
pseudouridine synthase RluD, pseudouridine synthases RluD, ribosomal large subunit pseudouridine synthase D, RluD, RluD pseudouridine synthase, sfhB, YfiI, YfiL
ECTree
5.4.99.23 23S rRNA pseudouridine1911/1915/1917 synthaseAdvanced search results
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results (Cloned
Cloned on EC 5.4.99.23 - 23S rRNA pseudouridine1911/1915/1917 synthase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
recombinant expression of chimeric enzyme mutants RluCD in Escherichia coli strains M15 of CD204, expression of chimeric pseudouridine synthase RluCD, under control of arabinose inducible araBAD promoter, interferes with ribosome formation in wild-type M15 strain and strain CD204 lacking RluC and RluD, while cells transformed with catalytically inactive RluCD (RluCD D139N) and empty vector (pQE60), used as a controls, represent a normal ribosomal profile in sucrose density gradient. Strain CD204 contains a mutant form of RF-2 (D131Y) that suppresses ribosome assembly defect caused by the deletion of the RluD. Strain rluD114 where yfiI (RluD) gene has been disrupted with Km cassette is used as a parent strain to construct strain CD204
wild-type and mutant enzymes D139T and D139N are overexpressed from plasmids
