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Literature summary for 5.4.99.23 extracted from

  • Gutgsell, N.S.; Del Campo, M.; Raychaudhuri, S.; Ofengand, J.
    A second function for pseudouridine synthases: A point mutant of RluD unable to form pseudouridines 1911, 1915, and 1917 in Escherichia coli 23S ribosomal RNA restores normal growth to an RluD-minus strain (2001), RNA, 7, 990-998.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes D139T and D139N are overexpressed from plasmids Escherichia coli

Protein Variants

Protein Variants Comment Organism
D139N mutation completely blocks pseudouridine formation in vivo and in vitro. The mutant rluD gene produces a protein capable of complete reversal of the growth defect (of Escherichia coli mutant with a truncation in the rluD gene) without concomitant pseudouridine formation Escherichia coli
D139T mutation completely blocks pseudouridine formation in vivo and in vitro. The mutant rluD gene produces a protein capable of complete reversal of the growth defect (of Escherichia coli mutant with a truncation in the rluD gene) without concomitant pseudouridine formation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
23S rRNA uridine1911/uridine1915/uridine1917 Escherichia coli
-
 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P33643
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
23S rRNA uridine1911/uridine1915/uridine1917
-
 Escherichia coli 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
 ?

Synonyms

Synonyms Comment Organism
RluD
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction a truncation mutant of the gene for RluD (responsible for synthesis of 23S rRNA pseudouridines 1911, 1915, and 1917) blocks pseudouridine formation and inhibits growth. RluD mutants D139T and D139N are completely inactive in vivo and in vitro. In vivo, the growth defect can be completely restored by transformation of an RluD-inactive strain with plasmids carrying genes for RluD D139T or RluD D139N. Pseudouridine sequencing of the 23S rRNA from these transformed strains demonstrates the lack of these pseudouridines. Pseudoreversion is not responsible because transformation with empty vector under identical conditions does not alter the growth rate Escherichia coli