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Literature summary for 5.4.99.23 extracted from

  • Raychaudhuri, S.; Conrad, J.; Hall, B.G.; Ofengand, J.
    A pseudouridine synthase required for the formation of two universally conserved pseudouridines in ribosomal RNA is essential for normal growth of Escherichia coli (1998), RNA, 4, 1407-1417.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
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 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
23S rRNA uridine1911/uridine1915/uridine1917 Escherichia coli
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 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P33643
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
23S rRNA uridine1911/uridine1915/uridine1917
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 Escherichia coli 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
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 ?
23S rRNA uridine1911/uridine1915/uridine1917 the enzyme is responsible in vivo for synthesis of the three pseudouridines clustered in a stem-loop at positions 1911, 1915, and 1917 of 23S RNA Escherichia coli 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
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 ?

Synonyms

Synonyms Comment Organism
RluD
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 Escherichia coli
sfhB
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 Escherichia coli
YfiL
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 Escherichia coli

General Information

General Information Comment Organism
malfunction the absence of RluD results in severe growth inhibition. Both the absence of pseudouridine and the growth defect can be reversed by insertion of a plasmid carrying the rluD gene into the mutant cell, clearly linking both effects to the absence of RluD. Growth inhibition may be due to the lack of one or more of the 23S RNA pseudouridines made by this synthase since pseudouridines 1915 and 1917 are universally conserved and are located in proximity to the decoding center of the ribosome where they could be involved in modulating codon recognition Escherichia coli