5.4.4.6: 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase

This is an abbreviated version!
For detailed information about 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase, go to the full flat file.

Reaction

Synonyms

7,8-diol synthase, 7,8-LDS, 7,8-linoleate diol synthase, EC 1.13.11.44

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.4 Transferring hydroxy groups

                5.4.4.6 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase

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Results	in table
3	Activating Compound
5	AA Sequence
5	Cloned(Commentary)
3	Cofactor
3	Inhibitors
4	kcat/KM [mM/s]
4	KM Value [mM]
1	Metals/Ions
3	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
22	Organism
1	Pathway
4	pH Optimum
1	pH Stability
1	pI Value
2	Posttranslational Modification
29	Protein Variants
5	Purification (Commentary)
1	Reaction
16	Reference
7	Source Tissue
4	Specific Activity [micromol/min/mg]
32	Substrates and Products (Substrate)
6	Subunits
16	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
4	Turnover Number [1/s]

pH Optimum

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pH Optimum on EC 5.4.4.6 - 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6.5

Colletotrichum gloeosporioides

A0A0S2SWE4

whole enzyme activity

741712

7 - 10

Gaeumannomyces graminis

-

broad optimum

285249

7.5

Colletotrichum gloeosporioides

A0A0S2SWE4

assay at

742730

additional information

Colletotrichum gloeosporioides

A0A0S2SWE4

the maximum activity for the conversion of linoleic acid to (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) by 8-dioxygenase in the N-terminal domain of 7,8-LDS is observed at pH 7.0 and 20°C, whereas that for the conversion of linoleic acid to (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by the whole enzyme is observed at pH 6.5 and 40°C

741712