5.3.3.14: trans-2-decenoyl-[acyl-carrier protein] isomerase

This is an abbreviated version!
For detailed information about trans-2-decenoyl-[acyl-carrier protein] isomerase, go to the full flat file.

Reaction

Synonyms

FabM

ECTree

     5 Isomerases

         5.3 Intramolecular oxidoreductases

             5.3.3 Transposing C=C bonds

                5.3.3.14 trans-2-decenoyl-[acyl-carrier protein] isomerase

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Results	in table
10266	AA Sequence
1	CAS Registry Number
1	Cloned(Commentary)
1	Crystallization (Commentary)
9	Inhibitors
4	Ki Value [mM]
2	KM Value [mM]
7	Molecular Weight [Da]
10	Organism
7	Pathway
34	PDB ID
1	pH Optimum
1	pH Range
1	Protein Variants
2	Purification (Commentary)
3	Reaction
10	Reference
2	Specific Activity [micromol/min/mg]
9	Substrates and Products (Substrate)
4	Subunits
1	Synonyms
1	Systematic Name
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 5.3.3.14 - trans-2-decenoyl-[acyl-carrier protein] isomerase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

cis-3-decenoyl-N-acetylcysteamine

trans-2-decenoyl-N-acetylcysteamine

Escherichia coli

-

-

5803

-

-

r

cis-beta,gamma-decenoyl-N-acetylcysteamine

?

Escherichia coli

-

-

5800

-

-

?

trans-2-decenoyl-(acyl-carrier protein)

cis-3-decenoyl-(acyl-carrier protein)

Escherichia coli

-

enzyme is equally active with acyl carrier protein derived from Escherichia coli, spinach or a protein A:acyl carrier protein fusion protein. With acyl carrier protein derived from Escherichia coli or from spinach, equilibrium results in equal amounts of trans-3- or cis-2-decenoyl-(acyl-carrier-protein), regardless of the initial substrate. With the fusion protein, yield is about 17% cis-3- and 49% trans-2-decenoyl-(acyl-carrier-protein)

5819

-

-

r

trans-2-decenoyl-N-acetylcysteamine

cis-3-decenoyl-N-acetylcysteamine

2 entries

trans-2-octenoyl-N-acetylcysteamine

cis-3-octenoyl-N-acetylcysteamine

Streptococcus pneumoniae

-

-

665480

-

-

?

additional information

?

-

2 entries

trans-2-decenoyl-N-acetylcysteamine

cis-3-decenoyl-N-acetylcysteamine

Escherichia coli

-

-

5819, 665451

-

-

r

trans-2-decenoyl-N-acetylcysteamine

cis-3-decenoyl-N-acetylcysteamine

Streptococcus pneumoniae

-

-

665480

-

-

?

additional information

?

-

Escherichia coli

-

enzyme activity depends more on acyl chain length than acyl carrier protein structure or origin

5819

-

-

?

additional information

?

-

Streptococcus pneumoniae

-

enzyme does not catalyze dehydration reaction of EC 4.2.1.60

665480

-

-

?