5.3.2.5: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase
This is an abbreviated version!
For detailed information about 2,3-diketo-5-methylthiopentyl-1-phosphate enolase, go to the full flat file.
Reaction
Synonyms
2,3-diketo-5-methylthiopentane 1-phosphate enolase, DK-MTP 1-P enolase, DK-MTP-1-P enolase, DKMTP-1-P enolase, DKMTP-1P enolase, MtnBD, MtnW, ribulose-1,5-bisphosphate carboxylase/oxygenase-like protein, RLP, RuBisCO-like protein, YkrW
ECTree
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Engineering
Engineering on EC 5.3.2.5 - 2,3-diketo-5-methylthiopentyl-1-phosphate enolase
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K122E
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the mutant fails to form a catalytic dimer and does not show any DK-MTP-1-P enolase activity
K122M
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the mutant fails to form a catalytic dimer and does not show any DK-MTP-1-P enolase activity
K122R
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the mutant has specific activity of 32% less than that of the wild type enzyme and a lower thermal stability than the wild type enzyme
K147A
site-directed mutagenesis, the mutant requires a 10fold greater concentration of protein for enolization of the natural substrate, reduced activity compared to the wild-type enzyme
K173A
site-directed mutagenesis, the mutant is able to catalyze enolization at approximately the same rate as the wild-type enzyme
K98A
site-directed mutagenesis, the mutant is unable to catalyze the enolase reaction
I19A
additional information
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a growth-defective mutant, in which the gene for the RLP is disrupted, is rescued by the gene for RuBisCO from the photosynthetic bacterium Rhodospirillum rubrum