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5.3.2.5: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

This is an abbreviated version!
For detailed information about 2,3-diketo-5-methylthiopentyl-1-phosphate enolase, go to the full flat file.

Word Map on EC 5.3.2.5

Reaction

5-(methylsulfanyl)-2,3-dioxopentyl phosphate
=
2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate

Synonyms

2,3-diketo-5-methylthiopentane 1-phosphate enolase, DK-MTP 1-P enolase, DK-MTP-1-P enolase, DKMTP-1-P enolase, DKMTP-1P enolase, MtnBD, MtnW, ribulose-1,5-bisphosphate carboxylase/oxygenase-like protein, RLP, RuBisCO-like protein, YkrW

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.2 Interconverting keto- and enol-groups
                5.3.2.5 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

Cloned

Cloned on EC 5.3.2.5 - 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Bacillus subtilis RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
-
expressed in Escherichia coli BL21 (DE3) pLysS cells
-
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene rbcLIV, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, cloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21
-
gene rlpA, form II RubisCO-like protein, cloning and expression in Escherichia coli strain DH5alpha
-
Geobacillus kaustophilis RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
-
Microcystis aeruginosa RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
-
overexpression in Escherichia coli
-
overexpression in Klebsiella pneumoniae strain CG253 leading to 5-6fold increased activity compared to the parent strain
-
Rhodopseudomonas palustris RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
-
Rhodospirillum rubrum RLPalpha-1 sequence determination and analysis, and phylogenetic analysis
-