5.3.2.5: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

This is an abbreviated version!
For detailed information about 2,3-diketo-5-methylthiopentyl-1-phosphate enolase, go to the full flat file.

Reaction

Synonyms

2,3-diketo-5-methylthiopentane 1-phosphate enolase, DK-MTP 1-P enolase, DK-MTP-1-P enolase, DKMTP-1-P enolase, DKMTP-1P enolase, MtnBD, MtnW, ribulose-1,5-bisphosphate carboxylase/oxygenase-like protein, RLP, RuBisCO-like protein, YkrW

ECTree

     5 Isomerases

         5.3 Intramolecular oxidoreductases

             5.3.2 Interconverting keto- and enol-groups

                5.3.2.5 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

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Results	in table
1038	AA Sequence
12	Cloned(Commentary)
1	Crystallization (Commentary)
44	General Information
1	Inhibitors
2	kcat/KM [mM/s]
2	KM Value [mM]
10	Metals/Ions
2	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
121	Organism
3	Pathway
3	pH Optimum
9	Protein Variants
5	Purification (Commentary)
9	Reaction
20	Reference
1	Specific Activity [micromol/min/mg]
27	Substrates and Products (Substrate)
9	Subunits
47	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]
2	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.3.2.5 - 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

activated wild-type enolase, carboxylated on Lys173, with Mg2+ alone, or Mg2+, and HCO3-, or Mg2+ and substrate 2,3-diketohexane 1-phosphate, and of the selenomethionine-substituted enzyme variant, hanging drop method, 10-15 mg/ml protein in 20 mM Tris-HCl, pH 7.9, 100 mM NaCl, and 5-10 mM MgCl2, 100 mM NaCl, and 1-5 mM NaHCO3, mixed with 24% PEG 3350, 0.1 M Tris-HCl, pH 8.5, and 0.2 M ammonium acetate as precipitant for the crystals of wild-type enzyme with Mg2+, or 25% PEG 3350, 0.1 M HEPES, pH 7.5, and 0.2 M ammonium acetate as precipitant for the other crystal variants, room temperature, 6 days, X-ray diffraction structure determination and analysis at 1.7 A resolution, molecular replacement

Geobacillus kaustophilus

Q5L1E2

678268