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5.1.99.1: methylmalonyl-CoA epimerase

This is an abbreviated version!
For detailed information about methylmalonyl-CoA epimerase, go to the full flat file.

Word Map on EC 5.1.99.1

Reaction

(R)-methylmalonyl-CoA
=
(S)-methylmalonyl-CoA

Synonyms

DL-Methylmalonyl-CoA racemase, DL-Methylmalonyl-coenzyme A racemase, MCE, MCE-1, MCEE, Methylmalonyl coenzyme A racemase, methylmalonyl-CoA 2-epimerase, methylmalonyl-CoA 2-racemase, methylmalonyl-CoA epimerase, Methylmalonyl-CoA racemase, methylmalonyl-coenzyme A epimerase, mm-CoA epimerase, MMCE, Msed_0639, Racemase, methylmalonyl coenzyme A, TTE0360

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.99 Acting on other compounds
                5.1.99.1 methylmalonyl-CoA epimerase

Crystallization

Crystallization on EC 5.1.99.1 - methylmalonyl-CoA epimerase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
at 2.0 A resolutionm, crystals of recombinant enzyme are grown at 20°C using the hanging-drop, vapor-diffusion method, drops consisted of 2 microl of protein solution and 2 microl of mother liquor (0.1 M citric acid, pH 2.6, 1.6 M (NH4)2SO4), crystals are obtained after 7-8 days growth, native and derivative crystals are soaked in 2 M Li2SO4
crystallization of native, Co2+-substituted and SeMet-substituted enzyme by hanging-drop vapour-diffusion method
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