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5.1.3.7: UDP-N-acetylglucosamine 4-epimerase

This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine 4-epimerase, go to the full flat file.

Word Map on EC 5.1.3.7

Reaction

UDP-N-acetyl-alpha-D-glucosamine
=
UDP-N-acetyl-alpha-D-galactosamine

Synonyms

Epimerase, uridine diphosphoacetylglucosamine, Galactowaldenase, GalE, galE-2, GalESp2, GNE, Gne epimerase, group 3 epimerase, KfoA, More, TM0509, TMGalE, UAE, UDP acetylglucosamine epimerase, UDP-galactose 4-epimerase, UDP-galactose-4-epimerase, UDP-GalNAc 4-epimerase, UDP-Glc(NAc) 4-epimerase, UDP-GlcNAc 4-epimerase, UDP-GlcNAc epimerase, UDP-GlcNAc/Glc 4-epimerase, UDP-hexose 4-epimerase, UDP-N-acetylglucosamine 4'-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-N-acetylglucosamine-4'-epimerase, UDP-sugar 4-epimerase, UDPGlcNAc 4-epimerase, Uridine 5'-diphospho-N-acetylglucosamine-4-epimerase, Uridine diphosphate N-acetylglucosamine-4-epimerase, Uridine diphosphoacetylglucosamine epimerase, WbgU, WbPP

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.7 UDP-N-acetylglucosamine 4-epimerase

Crystallization

Crystallization on EC 5.1.3.7 - UDP-N-acetylglucosamine 4-epimerase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant mutant H305A enzyme, best by microbatch under oil set up and hanging drop vapor diffusion method, from a solution containing 25% PEG 3350, 0.2 M ammonium sulfate, cryoprotection by 15-20% glycerol, X-ray diffraction structure determination and analysis at 2.10 A resolution
-
microbatch under oil method, using 250 mM ammonium sulfate, 100 mM Bis-Tris pH 6.5, (25% w/v) PEG 3350
hanging drop vapor diffusion method, crystal structure of the enzyme in complex with cofactor and either UDP-glucose or UDP-N-acetyl-D-galactosamine, determined at 2.5 and 2.1 A, crystals belong to the space group P222(1), with unit cell dimensions of a = 61 A, b = 96 A, and c = 140 A
-
hanging drop vapor diffusion method, crystal structure of the enzyme (TMGalE) and the enzyme bound to UDP-glucose is determined at resolutions of 1.9 A and 2.0 A, respectively
purified recombinant unbound enzyme TMGalE, and TMGalE bound to UDP-Glc, X-ray diffraction structure determination and analysis at 1.9-2.0 A resolution