Literature summary for 5.1.3.7 extracted from

Ishiyama, N.; Creuzenet, C.; Lam, J.S.; Berghuis, A.M.
Crystal structure of WbpP, a genuine UDP-N-acetylglucosamine 4-epimerase from Pseudomonas aeruginosa: substrate specificity in udp-hexose 4-epimerases (2004), J. Biol. Chem., 279, 22635-22642.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, crystal structure of the enzyme in complex with cofactor and either UDP-glucose or UDP-N-acetyl-D-galactosamine, determined at 2.5 and 2.1 A, crystals belong to the space group P222(1), with unit cell dimensions of a = 61 A, b = 96 A, and c = 140 A	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
A209H	unlike the wild-type enzyme the mutant enzyme is more efficient in catalyzing the reaction with the non-acetylated hexoses UDP-glucose and UDP-galactose than in catalyzing epimerization of UDP-N-acetyl-D-glucosamine and UDP-N-acetyl-D-galactosamine	Pseudomonas aeruginosa
S306Y	completely inactive mutant enzyme	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	serotype O6	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-D-glucose	unlike the wild-type enzyme the mutant enzyme is more efficient in catalyzing the reaction with the non-acetylated hexoses UDP-Glc and UDP-Gal than in catalyzing epimerization of UDP-N-acetyl-D-glucosamine and UDP-N-acetyl-D-galactosamine	Pseudomonas aeruginosa	UDP-D-galactose	-	r
UDP-N-acetyl-D-glucosamine	wild-type enzyme is more efficient in catalyzing epimerization of UDP-N-acetyl-D-glucosamine and UDP-N-acetyl-D-galactosamine than in catalyzing the epimerization of UDP-glucose and UDP-galactose	Pseudomonas aeruginosa	UDP-N-acetyl-D-galactosamine	-	r

Subunits

Subunits	Comment	Organism
homodimer	-	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
WbPP	-	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)