5.1.3.30: D-psicose 3-epimerase
This is an abbreviated version!
For detailed information about D-psicose 3-epimerase, go to the full flat file.
Word Map on EC 5.1.3.30
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5.1.3.30
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d-fructose
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tumefaciens
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agrobacterium
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d-tagatose
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d-allulose
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clostridium
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bioconversion
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synthesis
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epimerization
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metal-dependent
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ruminococcus
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cellulolyticum
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low-calorie
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ketose
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sweetener
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reusable
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d-sorbose
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izumoring
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ketohexose
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ni-affinity
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food-grade
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low-energy
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reusability
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noncharacterized
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bolteae
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fermentor
- 5.1.3.30
- d-fructose
- tumefaciens
- agrobacterium
- d-tagatose
- d-allulose
- clostridium
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bioconversion
- synthesis
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epimerization
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metal-dependent
- ruminococcus
- cellulolyticum
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low-calorie
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ketose
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sweetener
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reusable
- d-sorbose
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izumoring
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ketohexose
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ni-affinity
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food-grade
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low-energy
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reusability
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noncharacterized
- bolteae
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fermentor
Reaction
Synonyms
ACL75304 protein, CB-DPEase, Ccel_0941, Clo1100_1157, CLOBOL_00069, CLOSCI_02528, Dosp-DPEase, DPE, DPEase, DTEase, RDPE, Trpr-DPEase
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Engineering
Engineering on EC 5.1.3.30 - D-psicose 3-epimerase
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additional information
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on the basis of the Izumoring strategy, D-allulose can be obtained from D-glucose by coupling D-glucose isomerase (GIase) and DPEase, with D-fructose as the intermediate. In this reaction system, D-fructose is firstly converted from D-glucose by GIase, and immediately isomerised to D-allulose by DPEase. Recombinant co-expression with GIase from Acidothermus cellulolyticus from plasmid pETDuet-Dosp-DPE/Acce-GI, optimization of the biotransformation consitions, method, overview. When the reactions reaches equilibrium under optimal conditions,the equilibrium ratio of D-glucose, D-fructose and D-allulose is approximately 6.5:7:3, respectively. The transformation rate is about 18%. The optimum pH of the Acce-GI/Dosp-DPE co-expression system is lower than that of the BGI/RDPE co-expression system, while the optimum temperature is higher than that of the BGI/RDPE co-expression system
additional information
enzyme overexpression for heterologous production of D-psicose from D-fructose, under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31. For high production of D-psicose, 216 g/L D-psicose are produced with 28.8% turnover yield at pH 6.5 and 55°C. The recombinant DPEase exhibits weak acid stability and thermostability and has a high affinity and turnover for the substrate D-fructose
additional information
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enzyme overexpression for heterologous production of D-psicose from D-fructose, under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31. For high production of D-psicose, 216 g/L D-psicose are produced with 28.8% turnover yield at pH 6.5 and 55°C. The recombinant DPEase exhibits weak acid stability and thermostability and has a high affinity and turnover for the substrate D-fructose
additional information
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enzyme overexpression for heterologous production of D-psicose from D-fructose, under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31. For high production of D-psicose, 216 g/L D-psicose are produced with 28.8% turnover yield at pH 6.5 and 55°C. The recombinant DPEase exhibits weak acid stability and thermostability and has a high affinity and turnover for the substrate D-fructose
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additional information
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high-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis, fed-batch fermentation in 7.5 l fermentor, overview
additional information
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immobilization of recombinant extracellular enzyme DPE onto anion exchange resin D301, DPE immobilization yield is 90%. D-Psicose is produced using the immobilized enzyme, separated by simulated moving bed chromatography (SMB), and purified by crystallization. The purity of the D-psicose crystals reaches 99.1%, method optimization, overview
additional information
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to improve the expression level of D-psicose 3-epimerase, the DPEase gene is fused with the promoter P43 to generate an expression cassette that introduced an identical cohesive end, and the resultant P43-DPE expression cassette is used as a monomeric fragment. The tandem repeats of the expression cassette are constructed by the isocaudamer method and integrated into the amyE gene locus of Bacillus subtilis by double-crossover. After DPEase is expressed in Bacillus subtilis, the antibiotic resistance gene is deleted via the Cre/lox system, thus generating food-grade strains. D-Psicose (D-allulose) production by freeze-dried enzyme powder, the optimal substrate concentration is 300 g/L of D-fructose, generating 26.67 g/l/h of D-allulose yield with an 8.89% conversion rate
additional information
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to improve the expression level of D-psicose 3-epimerase, the DPEase gene is fused with the promoter P43 to generate an expression cassette that introduced an identical cohesive end, and the resultant P43-DPE expression cassette is used as a monomeric fragment. The tandem repeats of the expression cassette are constructed by the isocaudamer method and integrated into the amyE gene locus of Bacillus subtilis by double-crossover. After DPEase is expressed in Bacillus subtilis, the antibiotic resistance gene is deleted via the Cre/lox system, thus generating food-grade strains. D-Psicose (D-allulose) production by freeze-dried enzyme powder, the optimal substrate concentration is 300 g/L of D-fructose, generating 26.67 g/l/h of D-allulose yield with an 8.89% conversion rate
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