5.1.3.30: D-psicose 3-epimerase
This is an abbreviated version!
For detailed information about D-psicose 3-epimerase, go to the full flat file.
Word Map on EC 5.1.3.30
-
5.1.3.30
-
d-fructose
-
tumefaciens
-
agrobacterium
-
d-tagatose
-
d-allulose
-
clostridium
-
bioconversion
-
synthesis
-
epimerization
-
metal-dependent
-
ruminococcus
-
cellulolyticum
-
low-calorie
-
ketose
-
sweetener
-
reusable
-
d-sorbose
-
izumoring
-
ketohexose
-
ni-affinity
-
food-grade
-
low-energy
-
reusability
-
noncharacterized
-
bolteae
-
fermentor
- 5.1.3.30
- d-fructose
- tumefaciens
- agrobacterium
- d-tagatose
- d-allulose
- clostridium
-
bioconversion
- synthesis
-
epimerization
-
metal-dependent
- ruminococcus
- cellulolyticum
-
low-calorie
-
ketose
-
sweetener
-
reusable
- d-sorbose
-
izumoring
-
ketohexose
-
ni-affinity
-
food-grade
-
low-energy
-
reusability
-
noncharacterized
- bolteae
-
fermentor
Reaction
Synonyms
ACL75304 protein, CB-DPEase, Ccel_0941, Clo1100_1157, CLOBOL_00069, CLOSCI_02528, Dosp-DPEase, DPE, DPEase, DTEase, RDPE, Trpr-DPEase
ECTree
5.1.3.30 D-psicose 3-epimeraseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 5.1.3.30 - D-psicose 3-epimerase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
20 - 60
-
activity of free DPE has small decreases after being maintained at 20-40°C, and has moderate decreases at 50-60°C, while the thermal stability of the immobilized DPE increases, particularly at 50°C
40
40 - 70
-
the purified recombinant enzyme retains 70% and 30% of its initial activity after 4 h of incubation at 40°C and 50°C, respectively, 55% activity remaining after 0.5 h at 60°C, complete inactivation after 0.5 h at 70°C
45
in the absence of Co2+, the activity is very stable below 45 °C but decreases at temperatures above 50 °C with increasing reaction times. When incubated with 0.4 mM Co2+, the half-lives (t1/2) of the enzyme are extended
50
55
60
70
-
recombinant enzyme in overexpressing Escherichia coli cells, pH 6.5, 9.3% activity remaining after 30 min, inactivation after 4 h
additional information
40
-
recombinant enzyme in overexpressing Escherichia coli cells, pH 6.5, 64.7%, activity remaining after 18 h
40
-
purified recombinant His6-tagged enzyme, over 75% activity remaining after 4 h
50
-
2 h, 42% loss of activity, thermostability property is almost not affected by the addition of Co2+
50
-
recombinant enzyme in overexpressing Escherichia coli cells, pH 6.5, 58.4% activity remaining after 18 h
50
in the absence of Co2+, the activity is very stable below 45 °C but decreases at temperatures above 50 °C with increasing reaction times. When incubated with 0.4 mM Co2+, the half-lives (t1/2) of the enzyme are extended
50
-
purified recombinant His6-tagged enzyme, 7% activity remaining after 4 h
55
the half-lives for the enzyme are 9.5 h and 24 min when incubated with and without Co2+ (0.1 mM)
60
-
the enzyme loses all of the initial activity after 0.5 h of exposure, thermostability property is almost not affected by the addition of Co2+
60
-
recombinant enzyme in overexpressing Escherichia coli cells, pH 6.5, 15.2% activity remaining after 18 h
60
the half-lives for the enzyme are 6.8 h and 10 min when incubated with and without Co2+ (0.1 mM)
additional information
-
Co2+ slightly improves the thermostability of the enzyme
additional information
-
the thermostability of the enzyme is enhanced by the addition of Mn2+
