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5.1.3.30: D-psicose 3-epimerase

This is an abbreviated version!
For detailed information about D-psicose 3-epimerase, go to the full flat file.

Word Map on EC 5.1.3.30

Reaction

D-psicose
=
D-fructose

Synonyms

ACL75304 protein, CB-DPEase, Ccel_0941, Clo1100_1157, CLOBOL_00069, CLOSCI_02528, Dosp-DPEase, DPE, DPEase, DTEase, RDPE, Trpr-DPEase

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.30 D-psicose 3-epimerase

Cloned

Cloned on EC 5.1.3.30 - D-psicose 3-epimerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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expressed in Escherichia coli
expressed with a C-terminal 6His-tag in Escherichia coli
expression in Escherichia coli
gene CLOBOL_00069, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli
gene dpe, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene dpe, recombinant food-grade overexpression of His-tagged enzyme in Bacillus pumilus using the P2 promoter, derived from a cell wall protein promoter of the host bacterium, the recombinant protein is soluble and bioactive, and is secreted to a high leve, subcloning in Escherichis coli strain DH5alphal
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gene rdpe, recombinant constitutive enzyme expression in Bacillus subtilis strains 1A751-SR, 1A751-GR, and 1A751S1-XR (from strain 1A751), in which two extracellular proteases (nprE, aprE) are deleted, Bacillus subtilis is transformed using the Paris method, screening and characterization of different inducible promoters, xylose-inducible expression system, method evaluation, detailed overview
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gene rdpe, recombinant expression in and secretion from Bacillus subtilis alanine racemase knockout (deletion of gene dal using Cre/lox system) strains 1A751D2R and 1A751D2C, construction of food-grade expression plasmids with auxotrophic marker, the food-grade plasmids pMA5-DAL-RDPE and pMA5-DAL are transformed into the food-grade host strain 1A751D2 with the deficiency of alanine racemase gene to generate the recombinant strain 1A751D2R and 1A751D2C, respectively. The extracellular activity of enzyme RDPE is gradually increased with the fermentation process, and the highest activity reaches 46 U/ml at 72 h, fed-batch fermentation, method evaluation, overview
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recombinant enzyme expression in Escherichia coli strain BL21(DE3), co-expression with D-glucose isomerase (GIase) from Acidothermus cellulolyticus from plasmid pETDuet-Dosp-DPE/Acce-GI
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recombinant expression of D-psicose 3-epimerase and display on the surface of Bacillus subtilis spores. To improve the expression level of D-psicose 3-epimerase, the DPEase gene is fused with the promoter P43 to generate an expression cassette that introduced an identical cohesive end, and the resultant P43-DPE expression cassette is used as a monomeric fragment. The tandem repeats of the expression cassette are constructed by the isocaudamer method and integrated into the amyE gene locus of Bacillus subtilis by double-crossover. After DPEase is expressed in Bacillus subtilis, the antibiotic resistance gene is deleted via the Cre/lox system, thus generating food-grade strains
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