5.1.1.7: diaminopimelate epimerase

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For detailed information about diaminopimelate epimerase, go to the full flat file.

Word Map on EC 5.1.1.7

5.1.1.7

meso-dap

racemase

peptidoglycan

l-lysine

drug development

ll-dap

plp-independent

meso-diaminopimelate

epimerization

d-cycloserine

stereoinversion

l,l-diaminopimelate

two-base

analysis

Reaction

Synonyms

CgDapF, DAP, DAP epimerase, DAP-epimerase, DapF, DapFCT, diaminopimelate epimerase, Diaminopimelic acid epimerase, Diaminopimelic epimerase, Epimerase, diaminopimelate, LL-Diaminopimelate epimerase, MtDapF, YddE

ECTree

5 Isomerases

5.1 Racemases and epimerases

5.1.1 Acting on amino acids and derivatives

5.1.1.7 diaminopimelate epimerase

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Results	in table
8	Activating Compound
37558	AA Sequence
13	Application
1	CAS Registry Number
12	Cloned(Commentary)
2	Cofactor
12	Crystallization (Commentary)
21	General Information
2	General Stability
1	IC50 Value
34	Inhibitors
12	KM Value [mM]
8	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
251	Organism
10	Pathway
38	PDB ID
5	pH Optimum
2	pH Range
4	pH Stability
32	Protein Variants
14	Purification (Commentary)
4	Reaction
5	Reaction Type
59	Reference
1	Source Tissue
7	Specific Activity [micromol/min/mg]
1	Storage Stability
60	Substrates and Products (Substrate)
7	Subunits
45	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 5.1.1.7 - diaminopimelate epimerase

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2 entries

by immobilized nickel metal-affinity chromatography using 5 ml HisTrap HP columns and by gel filtration as His-tagged enzyme

Escherichia coli

701554

by using nickel-chelate affinity chromatography

Mycobacterium tuberculosis

partial

protein is applied to a Ni21-primed chelating sepharose column, and DapF-containing eluate, fractions are dialysed and further purified by anion exchange chromatography on a HiTrap Q Sepharose FF column, representing a yield of 1 mg/L culture

Mycobacterium tuberculosis

673738

recombinant His6-tagged enzyme from Escherichia coli strain SoluBL21 by nickel affinity chromatography, tag cleavage by thrombin, another step of nickel affinity chromatography, ultrafiltration, and gel filtration

Acinetobacter baumannii

726632

recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS/pET15b by nickel affinity chromatography

Kitasatospora setae

E4NI20

728008

recombinant seleno-methionine substituted wild-type and mutant enzymes from Escherichia coli strain B834(DE3)

Corynebacterium glutamicum

Q8NP73

749346