Application | Comment | Organism |
---|---|---|
drug development | the enzme is an attractive target to facilitate development of antibacterial agents | Kitasatospora setae |
Cloned (Comment) | Organism |
---|---|
gene dapF1, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS/pET15b | Kitasatospora setae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | inactivation by 50% acetone | Kitasatospora setae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-Diaminoheptanedioate | Kitasatospora setae | - |
meso-Diaminoheptanedioate | - |
r | |
LL-2,6-Diaminoheptanedioate | Kitasatospora setae KM-6054 | - |
meso-Diaminoheptanedioate | - |
r |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | inactivation by 50% acetone | Kitasatospora setae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora setae | E4NI20 | gene dapF1; gene dapF1 | - |
Kitasatospora setae KM-6054 | E4NI20 | gene dapF1; gene dapF1 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS/pET15b by nickel affinity chromatography | Kitasatospora setae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.61 | - |
purified recombinant His-tagged enzyme, substrate meso-diaminoheptanedioate, pH 6.8, 30°C | Kitasatospora setae |
4.93 | - |
purified recombinant His-tagged enzyme, substrate LL-2,6-diaminoheptanedioate, pH 6.8, 30°C | Kitasatospora setae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-Diaminoheptanedioate | - |
Kitasatospora setae | meso-Diaminoheptanedioate | - |
r | |
LL-2,6-Diaminoheptanedioate | - |
Kitasatospora setae KM-6054 | meso-Diaminoheptanedioate | - |
r | |
additional information | no activity with DD-2,6-diaminoheptanedioate. Development of a simple method using thin-layer chromatography, in methanol/water (64:36) and with ninhydrin detection, and chiral column chromatography to allow preparation of pure diaminopimelate isomers and detect products, respectively, overview | Kitasatospora setae | ? | - |
? | |
additional information | no activity with DD-2,6-diaminoheptanedioate. Development of a simple method using thin-layer chromatography, in methanol/water (64:36) and with ninhydrin detection, and chiral column chromatography to allow preparation of pure diaminopimelate isomers and detect products, respectively, overview | Kitasatospora setae KM-6054 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAP epimerase | - |
Kitasatospora setae |
DapF | - |
Kitasatospora setae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Kitasatospora setae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Kitasatospora setae |
General Information | Comment | Organism |
---|---|---|
physiological function | diaminopimelate isomers are not only intermediates of the lysine biosynthesis diaminopimelate pathway but also essential components of bacterial peptidoglycan | Kitasatospora setae |