Literature summary for 5.1.1.7 extracted from

Usha, V.; Dover, L.G.; Roper, D.I.; Fuetterer, K.; Besra, G.S.
Structure of the diaminopimelate epimerase DapF from Mycobacterium tuberculosis (2009), Acta Crystallogr. Sect. D, 65, 383-387.

Search for more literature for 5.1.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant protein is expressed as His-tegged enzyme in BL21(DE3) Escherichia coli cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of the ligand-free form refines to a resolution of 2.6 A, 2.5 mM dithiothreitol is present in the crystal drop	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
LL-2,6-diaminoheptanedioate	Mycobacterium tuberculosis	stereo-inversion	meso-diaminopimelate	the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan	?
LL-2,6-diaminoheptanedioate	Mycobacterium tuberculosis H37Rv	stereo-inversion	meso-diaminopimelate	the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WP19	-	-
Mycobacterium tuberculosis H37Rv	P9WP19	-	-

Purification (Commentary)

Purification (Comment)	Organism
by using nickel-chelate affinity chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
LL-2,6-diaminoheptanedioate	stereo-inversion	Mycobacterium tuberculosis	meso-diaminopimelate	the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan	?
LL-2,6-diaminoheptanedioate	stereo-inversion	Mycobacterium tuberculosis H37Rv	meso-diaminopimelate	the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan	?

Synonyms

Synonyms	Comment	Organism
DAP epimerase	-	Mycobacterium tuberculosis
DapF	-	Mycobacterium tuberculosis
diaminopimelate epimerase	-	Mycobacterium tuberculosis
MtDapF	-	Mycobacterium tuberculosis

General Information

General Information	Comment	Organism
physiological function	synthesis of L-lysine	Mycobacterium tuberculosis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)