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5.1.1.3: glutamate racemase

This is an abbreviated version!
For detailed information about glutamate racemase, go to the full flat file.

Word Map on EC 5.1.1.3

Reaction

L-glutamate
=
D-glutamate

Synonyms

AAR, BAS0806, BAS4379, BcGR, BsGR, BsRacE, CBL/ALR, cystathionine beta-lyase, D-glutamate racemase, DapF, FnGR, GBAA_0847, GBAA_4717, GLR, GluR, glutamate racemase, glutamic acid racemases, GRL, HpMurI, MetC, More, MurI, RACE, RacE1, RacE2, Racemase, glutamate, Rv1338, TmCBL, wMelCBL

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.3 glutamate racemase

Temperature Stability

Temperature Stability on EC 5.1.1.3 - glutamate racemase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
fully stable up to
40
-
purified recombinant enzyme, pH 8.0, moderately stable preserving more than 80% of initial activity after 2 h at up to 40°C, but rapidly loses all activity at higher temperatures
49
-
Tm value (midpoint of thermal unfolding) of the wild type enzyme
50
pH 8.0, 0.1 M Tris-HCl buffer containing 10% glycerol, retains 90% of its activity after 10 min
51
-
Tm value (midpoint of thermal unfolding) of the R25A mutant
60
-
pH 7, 60 min, about 25% loss of activity of soluble and immobilized enzyme
80
-
pH 7, 60 min, about 20% loss of activity of immobilized enzyme, about 70% loss of activity of soluble enzyme
90
-
pH 7, 60 min, 50% loss of activity of immobilized enzyme, about 75% loss of activity of soluble enzyme