5.1.1.3: glutamate racemase
This is an abbreviated version!
For detailed information about glutamate racemase, go to the full flat file.
Word Map on EC 5.1.1.3
-
5.1.1.3
-
peptidoglycan
-
l-glutamate
-
cofactor-independent
-
drug development
-
pediococcus
-
poly-gamma-glutamate
-
pyrophilus
-
pentosaceus
-
fermenti
-
ciceri
-
stereoinversion
-
medicine
-
synthesis
- 5.1.1.3
- peptidoglycan
- l-glutamate
-
cofactor-independent
- drug development
- pediococcus
-
poly-gamma-glutamate
- pyrophilus
- pentosaceus
- fermenti
-
ciceri
-
stereoinversion
- medicine
- synthesis
Reaction
Synonyms
AAR, BAS0806, BAS4379, BcGR, BsGR, BsRacE, CBL/ALR, cystathionine beta-lyase, D-glutamate racemase, DapF, FnGR, GBAA_0847, GBAA_4717, GLR, GluR, glutamate racemase, glutamic acid racemases, GRL, HpMurI, MetC, More, MurI, RACE, RacE1, RacE2, Racemase, glutamate, Rv1338, TmCBL, wMelCBL
ECTree
Advanced search results
KCat KM Value
KCat KM Value on EC 5.1.1.3 - glutamate racemase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
0.09
L-2-aminoadipic acid
His-tagged recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)
0.0031
-
recombinant wild type enzyme, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
0.0035
D-glutamate
-
mutant K106A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
0.0059
D-glutamate
-
mutant Y221A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
0.006
D-glutamate
-
mutant Q86A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
0.0062
D-glutamate
-
mutant R214A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
0.0083
D-glutamate
-
mutant K29A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
0.0125
D-glutamate
-
mutant P99A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
0.0139
D-glutamate
-
mutant R214A/K106A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
0.015
D-glutamate
-
mutant R25A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate
1.5
D-glutamate
His-tagged recombinant BsGR mutant V149A, in potassium phosphate buffer (10 mM, pH 8.0)
4
D-glutamate
His-tagged recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)
5
D-glutamate
His-tagged recombinant BsGR, in potassium phosphate buffer (10 mM, pH 8.0)
15
D-glutamate
recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)
0.0045
-
mutant Q86A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.0053
L-glutamate
-
recombinant wild type enzyme, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.0056
L-glutamate
-
mutant K106A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.007
L-glutamate
-
mutant R214A/K106A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.0071
L-glutamate
-
mutant Y221A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.0098
L-glutamate
-
mutant R214A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.0111
L-glutamate
-
mutant P99A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.0118
L-glutamate
-
mutant K29A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.014
L-glutamate
-
mutant R25A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol
0.092
L-glutamate
recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer
0.71
L-glutamate
recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer
1.8
L-glutamate
His-tagged recombinant BsGR mutant V149A, in potassium phosphate buffer (10 mM, pH 8.0)
4.6
L-glutamate
His-tagged recombinant BsGR, in potassium phosphate buffer (10 mM, pH 8.0)
15
L-glutamate
His-tagged recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)
17
L-glutamate
recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)