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4.2.3.39: epi-cedrol synthase

This is an abbreviated version!
For detailed information about epi-cedrol synthase, go to the full flat file.

Word Map on EC 4.2.3.39

Reaction

(2E,6E)-farnesyl diphosphate
+
H2O
=
8-epi-cedrol
 +
diphosphate

Synonyms

8-epicedrol synthase, EPCS, epicedrol synthase, sesquiterpene cyclase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.39 epi-cedrol synthase

Cloned

Cloned on EC 4.2.3.39 - epi-cedrol synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from a cDNA library, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue
expressed in Escherichia coli BL21(DE3) cells
functional expression in Saccharomyces cerevisiae, native mating type alpha yeast strain JBY574 and constructed strain EHY42, leading to production of sesquiterpenes in yeast. Expressing epi-cedrol synthase in the upc2-1 mutant CJ-2A actually decreases foreign sesquiterpene yields relative to wild-type strain. FPP is apparently less accessible to the epi-cedrol synthase in the upc2-1 mutant, overview. Mating type influences foreign sesquiterpene production, overview
-
homology-based cloning from a cDNA library, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strains DH5alpha and BL21(DE3), respectively