4.2.3.135: DELTA6-protoilludene synthase
This is an abbreviated version!
For detailed information about DELTA6-protoilludene synthase, go to the full flat file.
Word Map on EC 4.2.3.135
Reaction
Synonyms
6-protoilludene synthase, DELTA6-protoilludene sesquiterpene synthase, Omp6, OMP7, PpSTS-08, protoilludene synthase, Stehi25180, Stehi64702, Stehi73029
ECTree
4.2.3.135 DELTA6-protoilludene synthaseAdvanced search results
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results (Engineering
Engineering on EC 4.2.3.135 - DELTA6-protoilludene synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A159E
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site-directed mutagenesis of Omp7, compared to wild-type, the mutant shows moderate reduction of activity with Mg2+ and Mn2+, slight to moderate reduction with Co2+, slightly reduced activity with Ni2+, and strong reduction of activity with Ca2+
S93E/A159E
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site-directed mutagenesis of Omp7, compared to wild-type, the mutant shows moderate reduction of activity with Co2+, Mg2+, and Mn2+, slightly reduced activity with Ni2+, and strong reduction of activity with Ca2+
T165A
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site-directed mutagenesis of Omp6, compared to wild-type, the mutant shows moderate reduction of activity with Mg2+ and Ni2+, slight to moderate reduction with Co2+, slightly reduced activity with Mn2+, and very strong reduction of activity with Ca2+
D111A
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site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows moderately reduced activity with Mg2+, slight to moderate reduction of activity with Mn2+, strong reduction of activity with Co2+, and no activity with Ca2+ and Ni2+
E117A
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site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows slight to moderate reduction of activity with Mg2+, strongly reduced activity with Ca2+ and Mn2+, very strong reduction of activity with Co2+, and no activity with Ni2+
E164A
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site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows moderate reduction of activity with Mg2+, strong reduction with Ca2+, Co2+, and Mn2+, and very strong reduction with Ni2+
E187A
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site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows slight to moderate reduction of activity with Mg2+, and no activity with Ca2+, Mn2+, Ni2+, and Co2+
E53A
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site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows slight reduction of activity with Mg2+, moderately reduced activity with Ca2+ and Mn2+, and no activity with Ni2+ and Co2+
additional information
additional information
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protoilludene is successfully produced in Escherichia coli by overexpression of a hybrid exogenous mevalonate pathway, endogenous farnesyl diphosphate synthase (IspA), and protoilludene synthase (OMP7) of Omphalotus olearius
additional information
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coexpression of protoilludene synthase PpSTS-08 and diverse PpCYPs in Saccharomyces cerevisiae by chromosomal integration of the PpSTS-08 expression cassette and subsequent transformation with expression plasmids of 184 PpCYPs previously constructed resulting in 184 distinct transformants that give simultaneous expression of PpSTS-08 with each of the PpCYPs
