Information on EC 4.2.3.135 - DELTA6-protoilludene synthase

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The expected taxonomic range for this enzyme is: Agaricomycetes

EC NUMBER
COMMENTARY hide
4.2.3.135
-
RECOMMENDED NAME
GeneOntology No.
DELTA6-protoilludene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = DELTA6-protoilludene + diphosphate
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, DELTA6-protoilludene-forming)
Isolated from the fungus Armillaria gallica. Delta6-Protoilludene is the first step in the biosynthesis of the melleolides.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
6-protoilludene is the exclusive reaction product of protoilludene synthase
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
DELTA6-protoilludene + diphosphate
show the reaction diagram
(2E,6E)farnesyl diphosphate
6-protoilludene + diphosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
highest activity in the presence of 5 mM MgCl2
MnCl2
-
inhibits activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
-
5% ethanol causes strongly reduced activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00152 - 0.00502
(2E,6E)-farnesyl diphosphate
0.00053
(2E,6E)farnesyl diphosphate
-
pH 7.2, 22C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0037 - 0.0063
(2E,6E)-farnesyl diphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.889 - 4.284
(2E,6E)-farnesyl diphosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
calculated from cDNA
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 40000, SDS-PAGE, x * 41070, calculated
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 2.9 A resolution, space group P21
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
-
only 50% activity
719998
8.5
-
only 50% activity
719998
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
nearly complete loss of activity above 35C
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expression in Escherichia coli
-