Information on EC 4.2.3.135 - DELTA6-protoilludene synthase

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The expected taxonomic range for this enzyme is: Agaricomycetes

EC NUMBER
COMMENTARY
4.2.3.135
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RECOMMENDED NAME
GeneOntology No.
DELTA6-protoilludene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate = DELTA6-protoilludene + diphosphate
show the reaction diagram
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-
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(2E,6E)-farnesyl diphosphate = DELTA6-protoilludene + diphosphate
show the reaction diagram
1,11-ring closure of (2E,6E)-farnsyl diphosphate yields a highly reactive trans-humulyl carbocation. A 1,2-hydride shift followed by further ring closures results in the protoilludyl cation, which undergoes a final deprotonation to give DELTA6-protoilludene
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, DELTA6-protoilludene-forming)
Isolated from the fungus Armillaria gallica. Delta6-Protoilludene is the first step in the biosynthesis of the melleolides.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6-protoilludene synthase
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-
-
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protoilludene synthase
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
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6-protoilludene is the exclusive reaction product of protoilludene synthase
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
DELTA6-protoilludene + diphosphate
show the reaction diagram
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-
single product
-
?
(2E,6E)-farnesyl diphosphate
DELTA6-protoilludene + diphosphate
show the reaction diagram
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main product for isoforms 25180, 64702, 73029. Isoforms 64702 and 73029 appear to be less specific than other previously described DELTA6-protoilludene synthases, and are capable of simultaneously producing a 1,10-cyclization-derived scaffold, beta-elemene, when expressed in a heterologous host
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?
(2E,6E)farnesyl diphosphate
6-protoilludene + diphosphate
show the reaction diagram
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-
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-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
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highest activity in the presence of 5 mM MgCl2
MnCl2
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inhibits activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ethanol
-
5% ethanol causes strongly reduced activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00152
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(2E,6E)-farnesyl diphosphate
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isoform 73029, pH 8.0, 21C
0.00191
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(2E,6E)-farnesyl diphosphate
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isoform 64702, pH 8.0, 21C
0.00502
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(2E,6E)-farnesyl diphosphate
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isoform 25180, pH 8.0, 21C
0.00053
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(2E,6E)farnesyl diphosphate
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pH 7.2, 22C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0037
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(2E,6E)-farnesyl diphosphate
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isoform 64702, pH 8.0, 21C
0.0044
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(2E,6E)-farnesyl diphosphate
-
isoform 25180, pH 8.0, 21C
0.0063
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(2E,6E)-farnesyl diphosphate
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isoform 73029, pH 8.0, 21C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.889
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(2E,6E)-farnesyl diphosphate
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isoform 25180, pH 8.0, 21C
81
1.951
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(2E,6E)-farnesyl diphosphate
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isoform 64702, pH 8.0, 21C
81
4.284
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(2E,6E)-farnesyl diphosphate
-
isoform 73029, pH 8.0, 21C
81
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45000
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calculated from cDNA
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
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x * 40000, SDS-PAGE, x * 41070, calculated
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
to 2.9 A resolution, space group P21
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pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.8
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-
only 50% activity
8.5
-
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only 50% activity
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
-
-
nearly complete loss of activity above 35C
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
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expression in Escherichia coli
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