Information on EC 4.2.3.135 - DELTA6-protoilludene synthase

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The expected taxonomic range for this enzyme is: Agaricomycetes

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
4.2.3.135
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
DELTA6-protoilludene synthase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = DELTA6-protoilludene + diphosphate
show the reaction diagram
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-
-
-
(2E,6E)-farnesyl diphosphate = DELTA6-protoilludene + diphosphate
show the reaction diagram
1,11-ring closure of (2E,6E)-farnsyl diphosphate yields a highly reactive trans-humulyl carbocation. A 1,2-hydride shift followed by further ring closures results in the protoilludyl cation, which undergoes a final deprotonation to give DELTA6-protoilludene
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SplaateReaction_Type,Organism,Commentary,Literature
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, DELTA6-protoilludene-forming)
Isolated from the fungus Armillaria gallica. Delta6-Protoilludene is the first step in the biosynthesis of the melleolides.
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6-protoilludene synthase
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-
-
-
protoilludene synthase
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-
SplaateCAS_Registry_Number,Commentary
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SplaateGeneral_Information, Organism, Commentary, Literature
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
6-protoilludene is the exclusive reaction product of protoilludene synthase
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
DELTA6-protoilludene + diphosphate
show the reaction diagram
-
-
single product
?
(2E,6E)-farnesyl diphosphate
DELTA6-protoilludene + diphosphate
show the reaction diagram
-
-
main product for isoforms 25180, 64702, 73029. Isoforms 64702 and 73029 appear to be less specific than other previously described DELTA6-protoilludene synthases, and are capable of simultaneously producing a 1,10-cyclization-derived scaffold, beta-elemene, when expressed in a heterologous host
?
(2E,6E)farnesyl diphosphate
6-protoilludene + diphosphate
show the reaction diagram
-
-
-
?
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
SplaateCofactor,Organism,Commentary,Literature,Filename
SplaateMetals_Ions,Organism,Commentary, Literature
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
-
highest activity in the presence of 5 mM MgCl2
MnCl2
-
inhibits activity
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ethanol
-
5% ethanol causes strongly reduced activity
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00152
(2E,6E)-farnesyl diphosphate
-
isoform 73029, pH 8.0, 21C
0.00191
(2E,6E)-farnesyl diphosphate
-
isoform 64702, pH 8.0, 21C
0.00502
(2E,6E)-farnesyl diphosphate
-
isoform 25180, pH 8.0, 21C
0.00053
(2E,6E)farnesyl diphosphate
-
pH 7.2, 22C
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0037
(2E,6E)-farnesyl diphosphate
Stereum hirsutum
-
isoform 64702, pH 8.0, 21C
0.0044
(2E,6E)-farnesyl diphosphate
Stereum hirsutum
-
isoform 25180, pH 8.0, 21C
0.0063
(2E,6E)-farnesyl diphosphate
Stereum hirsutum
-
isoform 73029, pH 8.0, 21C
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.889
(2E,6E)-farnesyl diphosphate
Stereum hirsutum
-
isoform 25180, pH 8.0, 21C
81
1.951
(2E,6E)-farnesyl diphosphate
Stereum hirsutum
-
isoform 64702, pH 8.0, 21C
81
4.284
(2E,6E)-farnesyl diphosphate
Stereum hirsutum
-
isoform 73029, pH 8.0, 21C
81
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45000
-
calculated from cDNA
719998
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 40000, SDS-PAGE, x * 41070, calculated
SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 2.9 A resolution, space group P21
-
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.8
-
only 50% activity
719998
8.5
-
only 50% activity
719998
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
-
nearly complete loss of activity above 35C
719998
SplaateGeneral_Stability, Organism, Literature
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
SplaateCommentary, Organism, Literature
SplaateCommentary, Organism, Literature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expression in Escherichia coli
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SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature