Information on EC 4.2.3.135 - DELTA6-protoilludene synthase

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The expected taxonomic range for this enzyme is: Agaricomycetes

EC NUMBER
COMMENTARY
4.2.3.135
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RECOMMENDED NAME
GeneOntology No.
DELTA6-protoilludene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = DELTA6-protoilludene + diphosphate
show the reaction diagram
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-
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(2E,6E)-farnesyl diphosphate = DELTA6-protoilludene + diphosphate
show the reaction diagram
1,11-ring closure of (2E,6E)-farnsyl diphosphate yields a highly reactive trans-humulyl carbocation. A 1,2-hydride shift followed by further ring closures results in the protoilludyl cation, which undergoes a final deprotonation to give DELTA6-protoilludene
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, DELTA6-protoilludene-forming)
Isolated from the fungus Armillaria gallica. Delta6-Protoilludene is the first step in the biosynthesis of the melleolides.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6-protoilludene synthase
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-
-
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
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6-protoilludene is the exclusive reaction product of protoilludene synthase
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
DELTA6-protoilludene + diphosphate
show the reaction diagram
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-
single product
-
?
(2E,6E)-farnesyl diphosphate
DELTA6-protoilludene + diphosphate
show the reaction diagram
-
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main product for isoforms 25180, 64702, 73029. Isoforms 64702 and 73029 appear to be less specific than other previously described DELTA6-protoilludene synthases, and are capable of simultaneously producing a 1,10-cyclization-derived scaffold, beta-elemene, when expressed in a heterologous host
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?
(2E,6E)farnesyl diphosphate
6-protoilludene + diphosphate
show the reaction diagram
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-
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
-
highest activity in the presence of 5 mM MgCl2
MnCl2
-
inhibits activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ethanol
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5% ethanol causes strongly reduced activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00152
(2E,6E)-farnesyl diphosphate
-
isoform 73029, pH 8.0, 21C
0.00191
(2E,6E)-farnesyl diphosphate
-
isoform 64702, pH 8.0, 21C
0.00502
(2E,6E)-farnesyl diphosphate
-
isoform 25180, pH 8.0, 21C
0.00053
(2E,6E)farnesyl diphosphate
-
pH 7.2, 22C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0037
(2E,6E)-farnesyl diphosphate
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isoform 64702, pH 8.0, 21C
0.0044
(2E,6E)-farnesyl diphosphate
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isoform 25180, pH 8.0, 21C
0.0063
(2E,6E)-farnesyl diphosphate
-
isoform 73029, pH 8.0, 21C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.889
(2E,6E)-farnesyl diphosphate
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isoform 25180, pH 8.0, 21C
81
1.951
(2E,6E)-farnesyl diphosphate
-
isoform 64702, pH 8.0, 21C
81
4.284
(2E,6E)-farnesyl diphosphate
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isoform 73029, pH 8.0, 21C
81
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45000
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calculated from cDNA
719998
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
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x * 40000, SDS-PAGE, x * 41070, calculated
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 2.9 A resolution, space group P21
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.8
-
only 50% activity
719998
8.5
-
only 50% activity
719998
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
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nearly complete loss of activity above 35C
719998
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expression in Escherichia coli
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