4.2.3.104: alpha-humulene synthase
This is an abbreviated version!
For detailed information about alpha-humulene synthase, go to the full flat file.
Word Map on EC 4.2.3.104
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4.2.3.104
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ginger
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terpenoids
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mevalonate
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shampoo
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germacrene
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synthesis
- 4.2.3.104
- ginger
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terpenoids
- mevalonate
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shampoo
- germacrene
- synthesis
Reaction
Synonyms
(E)-beta-caryophyllene/alpha-humulene synthase, alpha-humulene synthase, beta-caryophyllene/alpha-humulene synthase, CAHS, Hs1, Hs2, HS3, hum, sesquiterpene synthase1, Sst1, TPS1, TPS12, TPS2, ZSS1, zssI
ECTree
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Substrates Products
Substrates Products on EC 4.2.3.104 - alpha-humulene synthase
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REACTION DIAGRAM
(2E)-geranyl diphosphate + H2O
geraniol + diphosphate
the enzyme produces limonene, linalool, alpha-terpineol, and geraniol from geranyl diphosphate. Geraniol is the main product
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(2Z)-neryl diphosphate
limonene + diphosphate
the enzyme produces linalool, alpha-terpineol, geraniol, alpha-terpinolene, p-menth-2-en-1-ol, piperitol, and nerol from neryl diphosphate. Limonene is the main product
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2 (2E,6E)-farnesyl diphosphate
beta-caryophyllene + alpha-humulene + 2 diphosphate
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4 (2E,6E)-farnesyl diphosphate
guaia-1(10),11-diene + guaia-1(5),11-diene + alpha-humulene + beta-elemene + 4 diphosphate
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products are 55% guaia-1(10),11-diene, 14% guaia-1(5),11-diene, 10% alpha-humulene, 10% beta-elemene, 6% alloaromadendrene and 5% beta-caryophyllene
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alpha-humulene + diphosphate
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(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
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enzyme produces beta-caryophyllene and alpha-humulene in a ratio of 71:29
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(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
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the main product is alpha-humulene, second product is beta-caryophyllene
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(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
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(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
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95% yield of alpha-humulene besides the minor product beta-caryophyllene (5%)
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the recombinant alpha-humulene synthase isozyme HS1 produces 94.6% alpha-humulene and 5.4% beta-caryophyllene
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additional information
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the recombinant alpha-humulene synthase isozyme HS1 produces 94.6% alpha-humulene and 5.4% beta-caryophyllene
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additional information
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the recombinant alpha-humulene synthase isozyme HS1 produces 94.6% alpha-humulene and 5.4% beta-caryophyllene
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additional information
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the recombinant alpha-humulene synthase isozyme HS2 produces 95.0% alpha-humulene and 5.0% beta-caryophyllene
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additional information
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the recombinant alpha-humulene synthase isozyme HS2 produces 95.0% alpha-humulene and 5.0% beta-caryophyllene
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additional information
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the recombinant alpha-humulene synthase isozyme HS2 produces 95.0% alpha-humulene and 5.0% beta-caryophyllene
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additional information
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the recombinant alpha-humulene synthase isozyme HS3 produces 93.5% alpha-humulene and 6.5% beta-caryophyllene
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additional information
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the recombinant alpha-humulene synthase isozyme HS3 produces 93.5% alpha-humulene and 6.5% beta-caryophyllene
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additional information
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the recombinant alpha-humulene synthase isozyme HS3 produces 93.5% alpha-humulene and 6.5% beta-caryophyllene
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additional information
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malachite green assay adapted to rapidly assay terpene synthase activity for activity determination, and GS-MS analysis
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additional information
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the recombinant alpha-humulene synthase produces 93.45% alpha-humulene and 5.5% beta-caryophyllene. Product determination by gas chromatography-flame ionization detection (GC-FID)
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