Information on EC 4.2.3.104 - alpha-humulene synthase

Word Map on EC 4.2.3.104
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.104
-
RECOMMENDED NAME
GeneOntology No.
alpha-humulene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = alpha-humulene + diphosphate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-caryophyllene biosynthesis
-
-
Sesquiterpenoid and triterpenoid biosynthesis
-
-
zerumbone biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (alpha-humulene-forming)
The enzyme from Zingiber zerumbet, shampoo ginger, also gives traces of beta-caryophyllene.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Solanum habrochaites
-
-
-
Manually annotated by BRENDA team
Scots pine
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
alpha-humulene synthase is a key enzyme of zerumbone biosynthesis
physiological function
after tobacco rattlevirus-based virus induced gene silencing, beta-elemene, guaia-1(5),11-diene and guaia-1(10),11-diene are undetectable in suppressed leaves
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
show the reaction diagram
(2E,6E)-farnesyl diphosphate
beta-caryophyllene + alpha-humulene + diphosphate
show the reaction diagram
-
-
-
?
4 (2E,6E)-farnesyl diphosphate
guaia-1(10),11-diene + guaia-1(5),11-diene + alpha-humulene + beta-elemene + 4 diphosphate
show the reaction diagram
-
products are 55% guaia-1(10),11-diene, 14% guaia-1(5),11-diene, 10% alpha-humulene, 10% beta-elemene, 6% alloaromadendrene and 5% beta-caryophyllene
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
show the reaction diagram
-
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0289
(2E,6E)-farnesyl diphosphate
30°C, pH not specified in the publication
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
-
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
lowest expression level
Manually annotated by BRENDA team
highest expression level; low expression
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64500
-
x * 64500, calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 64500, calculated from amino acid sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, gel filtration
-
nickel affinity column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) and JM109 cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21-CodonPlus (DE3) cells
-
expressed in Escherichia coli Rosetta-gami 2 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Diprion pini oviposition enhances the transcription of sesquiterpene synthase TPS1 by 17.5fold. The transcripts TPS1 and TPS2 are induced in combinations of pine and sawfly species only when the egg-laden needles are attractive to Closterocerus ruforum, which parasitizes sawfly eggs
-
expression is induced upon wounding or methyljasmonate treatment; expression is induced upon wounding or methyljasmonate treatment
in the presence of 1 mg/ml lithium acetoacetate, a 13.6fold increase in alpha-humulene production occurs compared with the control
-
the enzyme is up-regulated in both leaves and rhizomes after treatment with methyl jasmonate
-
the transcript of ZZS1 gene increases very rapidly from June 12 to June 28, a period of strong temperature increase in 2007. Transcript level remains high throughout the summer and autumn, but declines significantly in December
-