4.2.3.104: alpha-humulene synthase

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For detailed information about alpha-humulene synthase, go to the full flat file.

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Synonyms

(E)-beta-caryophyllene/alpha-humulene synthase, alpha-humulene synthase, beta-caryophyllene/alpha-humulene synthase, CAHS, Hs1, Hs2, HS3, hum, sesquiterpene synthase1, Sst1, TPS1, TPS12, TPS2, ZSS1, zssI

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.104 alpha-humulene synthase

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Results	in table
20	AA Sequence
1	Application
11	Cloned(Commentary)
6	Expression
7	General Information
7	KM Value [mM]
1	Localization
1	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
14	PDB ID
2	pH Optimum
1	pH Range
3	pI Value
1	Posttranslational Modification
4	Protein Variants
4	Purification (Commentary)
1	Reaction
16	Reference
16	Source Tissue
3	Specific Activity [micromol/min/mg]
20	Substrates and Products (Substrate)
2	Subunits
26	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Turnover Number [1/s]

Cloned

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Cloned on EC 4.2.3.104 - alpha-humulene synthase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli BL21 (DE3) and JM109 cells

Zingiber zerumbet

-

690593

expressed in Escherichia coli BL21(DE3) cells

Zingiber zerumbet

-

717468

expressed in Escherichia coli BL21-CodonPlus (DE3) cells

Zingiber zerumbet

-

718244

expressed in Escherichia coli Rosetta-gami 2 cells

Solanum lycopersicum

D5KXD2

718233

gene HS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli

Aquilaria crassna

A0A142F308, A0A142F309, A0A142F310

748440

gene HS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli

Aquilaria crassna

A0A142F308, A0A142F309, A0A142F310

748440

gene HS3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli

Aquilaria crassna

A0A142F308, A0A142F309, A0A142F310

748440

gene ZSS1 or hum, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) under control of the lac promoter

Zingiber zerumbet

B1B1U3

746789

gene ZSS1 or HUM, recombinant His-tagged enzyme expression from codon-optimized alpha-humulene synthase gene in Escherichia coli strain BL21(DE3)

Zingiber zerumbet

B1B1U3

747656

gene zssI, recombinant coexpression of alpha-humulene synthase of Zingiber zerumbet from codon-optimized gene zssI with farnesyl diphosphate (FPP) synthase from Saccharomyces cerevisiae leads to concentrations of up to 18 mg/l alpha-humulene in Methylobacterium extorquens strain AM1, cloning scheme comprising genes zssI and ERG20

Zingiber zerumbet

B1B1U3

748552

gene ZSSI, recombinant coexpression of alpha-humulene synthase of Zingiber zerumbet with alpha-humulene 8-hydroxylase (CYP71BA1) and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana in Saccharomyces cerevisiae strain LW16, subcloning and expression in Escherichia coli strain trans-T1

Zingiber zerumbet

B1B1U3

748561