4.2.2.5: chondroitin AC lyase
This is an abbreviated version!
For detailed information about chondroitin AC lyase, go to the full flat file.
Word Map on EC 4.2.2.5
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4.2.2.5
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glycosaminoglycans
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proteoglycans
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dermatan
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heparan
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hyaluronic
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hyaluronidase
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glucuronic
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heparinum
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flavobacterium
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heparitinase
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6-sulfate
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lyases
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iduronic
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4-sulphate
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glca
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cetylpyridinium
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hexasaccharide
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monosulfated
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cuprolinic
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3hglucosamine
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galactosaminoglycans
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aurescens
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undersulfated
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oversulfated
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keratanase
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35ssulfate
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analysis
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medicine
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synthesis
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trisulfated
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blue-positive
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chondroitin-4-sulfate
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food industry
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cupromeronic
- 4.2.2.5
- glycosaminoglycans
- proteoglycans
- dermatan
- heparan
-
hyaluronic
- hyaluronidase
-
glucuronic
- heparinum
- flavobacterium
- heparitinase
- 6-sulfate
- lyases
-
iduronic
-
4-sulphate
-
glca
-
cetylpyridinium
- hexasaccharide
-
monosulfated
-
cuprolinic
-
3hglucosamine
- galactosaminoglycans
- aurescens
-
undersulfated
-
oversulfated
- keratanase
-
35ssulfate
- analysis
- medicine
- synthesis
-
trisulfated
-
blue-positive
- chondroitin-4-sulfate
- food industry
-
cupromeronic
Reaction
= n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine +
Synonyms
A-Chase, AC I lyase, c-ACI, c-ACII, ChnAC, chon-AC-lyase, ChonAC, chondroitin AC eliminase, chondroitin AC I lyase, Chondroitin AC lyase, chondroitin ACII lyase, chondroitin lyase AC, chondroitin sulfate lyase, chondroitinase, chondroitinase AC, chondroitinase ACI, chondroitinase ACII, ChSase-AC, EC 4.2.99.6, F-Chase, lyase, chondroitin AC, Pedsa_3808, PL8A
ECTree
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Engineering
Engineering on EC 4.2.2.5 - chondroitin AC lyase
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I236T
H225A
site-directed mutagenesis, active site residue, inactive mutant
R288A
site-directed mutagenesis, active site residue, nearly inactive mutant
R292A
Y234F
additional information
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mutation introduced to render the enzyme identical to ACII from Arthrobacter aurescens IAM 110 65. Mutant behaves similar to wild-type
I236T
Paenarthrobacter aurescens 161MFSha2.1
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mutation introduced to render the enzyme identical to ACII from Arthrobacter aurescens IAM 110 65. Mutant behaves similar to wild-type
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site-directed mutagenesis, substrate recognition residue, mutant with residual activity
R292A
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site-directed mutagenesis, substrate recognition residue, reduced activity, action pattern is neither purely random endolytic nor purely random exolytic
site-directed mutagenesis, active site residue, nearly inactive mutant
a vector is engineered containing the gene for Flavobacterium heparinum chondroitinase AC for expression in adult bone marrow-derived cells which are then transplanted into an injury site in the CNS. Expression and secretion of active chondroitinase AC is observed in vitro using transfected Chinese hamster ovarian and gliosarcoma cells and in vivo by immunohistochemistry analysis which show degraded chondroitin sulfate coinciding with the location of transfected bone marrow-derived cells. Immunolabelling of the axonal growth-associated protein GAP-43 is observed in vivo and coincides with the location of degraded chondroitin sulfate
additional information
-
a vector is engineered containing the gene for Flavobacterium heparinum chondroitinase AC for expression in adult bone marrow-derived cells which are then transplanted into an injury site in the CNS. Expression and secretion of active chondroitinase AC is observed in vitro using transfected Chinese hamster ovarian and gliosarcoma cells and in vivo by immunohistochemistry analysis which show degraded chondroitin sulfate coinciding with the location of transfected bone marrow-derived cells. Immunolabelling of the axonal growth-associated protein GAP-43 is observed in vivo and coincides with the location of degraded chondroitin sulfate