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4.2.2.17: inulin fructotransferase (DFA-I-forming)

This is an abbreviated version!
For detailed information about inulin fructotransferase (DFA-I-forming), go to the full flat file.

Word Map on EC 4.2.2.17

Reaction

[inulin]n
=
[inulin]n-2
 +
alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride

Synonyms

EC 2.4.1.200, IFTase, inulin D-fructosyl-D-fructosyltransferase (1,2':2',1-dianhydride-forming), inulin fructotransferase (depolymerizing, difructofuranose-1,2':2',1-dianhydride-forming), inulin fructotransferase (DFA I-producing), inulin fructotransferase (DFA-I-producing), NBCG_01407, Noca_0068, UG56_016290

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.17 inulin fructotransferase (DFA-I-forming)

Engineering

Engineering on EC 4.2.2.17 - inulin fructotransferase (DFA-I-forming)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A257S
mutation of residue in central tunnel, no significant change of temperature stability
A257S/G281S G281S
mutation of residues in central tunnel, increase in temperature stability by 3 degrees
A257T
mutation of residue in central tunnel, 2.8 degrees decrease of temperature stability
D183A
-
complete loss of activity
D183N
-
complete loss of activity
E194A
-
complete loss of activity
E194N
-
complete loss of activity
G121A/T122L
148.7% of wild-type activity, about 4.5 degrees increase in temperature stability
G236S/G281S/A257S/T313S/A314S
140.5% of wild-type activity, increase in temperature stability by 3.5 degrees
G281S
mutation of residue in central tunnel, 76% of wild-type activity, increase in temperature stability by 3 degrees
G281T
mutation of residue in central tunnel, no significant change of temperature stability
I80A
11.6% of wild-type activity, about 2 degrees increase in temperature stability
I80E
15.7% of wild-type activity, about 1.5 degrees increase in temperature stability
I80S
41.4% of wild-type activity, about 1 degrees increase in temperature stability
D183A
-
complete loss of activity
-
D183N
-
complete loss of activity
-
E194A
-
complete loss of activity
-
E194N
-
complete loss of activity
-