Information on EC 4.2.2.17 - inulin fructotransferase (DFA-I-forming)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Arthrobacter

EC NUMBER
COMMENTARY hide
4.2.2.17
-
RECOMMENDED NAME
GeneOntology No.
inulin fructotransferase (DFA-I-forming)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride (DFA I) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-fructosyl-D-fructofuranosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2->1)-beta-D-fructan lyase (alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,1'-dianhydride-forming)
This enzyme, like EC 4.2.2.16 [levan fructotransferase (DFA-IV-forming)] and EC 4.2.2.18 [inulin fructotransferase (DFA-III-forming)] eliminates the fructan chain from the terminal disaccharide leaving a difructose dianhydride. These enzymes have long been known as fructotransferases, so this is retained in the accepted name. Since the transfer is intramolecular, the reaction is an elimination and, hence, the enzyme is a lyase, belonging in EC 4.
CAS REGISTRY NUMBER
COMMENTARY hide
125008-19-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MC12493
-
-
Manually annotated by BRENDA team
Arthrobacter MC12493
MC12493
-
-
Manually annotated by BRENDA team
Arthrobacter ureafaciens
Arthrobacter ureafaciens A51-1
strain A51-1
-
-
Manually annotated by BRENDA team
Arthrobacter ureafaciens a62-1
strain a62-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe3+
-
1 mM, strong
Hg2+
-
1 mM, strong
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
slight stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
inulin
Arthrobacter ureafaciens
-
pH 5.5, 45C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
127
Arthrobacter ureafaciens
-
-
172
Arthrobacter ureafaciens
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
Arthrobacter ureafaciens
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 8
-
10% of maximal activity at pH 3.5, 25% of maximal activity at pH 8.0
4.5 - 7
Arthrobacter ureafaciens
-
pH 4.5: about 50% of maximal activity, pH 7.0: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
Arthrobacter ureafaciens
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
80% of maximal activity at 30C, 20% of maximal activity at 65C
30 - 60
Arthrobacter ureafaciens
-
30C: about 70% of maximal activity, 60C: about 45% of maximal activity
30 - 65
Arthrobacter ureafaciens
-
30C: about 80% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
-
SDS-PAGE
40000
-
SDS-PAGE and gel filtration
41600
-
amino acid analysis
46000
-
gel filtration
60000
Arthrobacter ureafaciens
-
gel filtration
61000
Arthrobacter ureafaciens
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
stable at 25C
644467
4
-
30C, 30 min., stable
644466
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
stable, rapid inactivation above 80C
70
-
pH 6, 30 min., 80% activity
85
Arthrobacter ureafaciens
-
pH 5.5, 1 h, complete inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Arthrobacter ureafaciens
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli MV1184
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition