Information on EC 4.2.2.17 - inulin fructotransferase (DFA-I-forming)

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The expected taxonomic range for this enzyme is: Arthrobacter

EC NUMBER
COMMENTARY
4.2.2.17
-
RECOMMENDED NAME
GeneOntology No.
inulin fructotransferase (DFA-I-forming)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride (DFA I) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D-fructosyl-D-fructofuranosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2->1)-beta-D-fructan lyase (alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,1'-dianhydride-forming)
This enzyme, like EC 4.2.2.16 [levan fructotransferase (DFA-IV-forming)] and EC 4.2.2.18 [inulin fructotransferase (DFA-III-forming)] eliminates the fructan chain from the terminal disaccharide leaving a difructose dianhydride. These enzymes have long been known as fructotransferases, so this is retained in the accepted name. Since the transfer is intramolecular, the reaction is an elimination and, hence, the enzyme is a lyase, belonging in EC 4.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
EC 2.4.1.200
-
-
formerly
-
inulin D-fructosyl-D-fructosyltransferase (1,2':2',1-dianhydride-forming)
-
-
-
-
inulin fructotransferase (depolymerizing, difructofuranose-1,2':2',1-dianhydride-forming)
-
-
-
-
inulin fructotransferase (DFA I-producing)
-
-
inulin fructotransferase (DFA I-producing)
Arthrobacter ureafaciens a62-1
-
-
-
inulin fructotransferase (DFA-I-producing)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
125008-19-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MC12493
-
-
Manually annotated by BRENDA team
Arthrobacter globiformis S14-3
S14-3
-
-
Manually annotated by BRENDA team
Arthrobacter MC12493
MC12493
-
-
Manually annotated by BRENDA team
strain A51-1
-
-
Manually annotated by BRENDA team
strain a62-1
-
-
Manually annotated by BRENDA team
Arthrobacter ureafaciens A51-1
strain A51-1
-
-
Manually annotated by BRENDA team
Arthrobacter ureafaciens a62-1
strain a62-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter, Arthrobacter MC12493
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter ureafaciens a62-1
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter ureafaciens A51-1
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter, Arthrobacter MC12493
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Fe3+
-
1 mM, strong
Hg2+
-
1 mM, strong
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
EDTA
-
slight stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4
inulin
-
pH 5.5, 45C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.5 - 8
-
10% of maximal activity at pH 3.5, 25% of maximal activity at pH 8.0
4.5 - 7
-
pH 4.5: about 50% of maximal activity, pH 7.0: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 60
-
30C: about 70% of maximal activity, 60C: about 45% of maximal activity
30 - 65
-
30C: about 80% of maximal activity
30
-
80% of maximal activity at 30C, 20% of maximal activity at 65C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Arthrobacter ureafaciens a62-1
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Arthrobacter globiformis S14-3
-
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
39000
-
SDS-PAGE
644465
40000
-
SDS-PAGE and gel filtration
644466
41600
-
amino acid analysis
644465
46000
-
gel filtration
644465, 644467
60000
-
gel filtration
664513
61000
-
gel filtration
664404
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
-
2 * 38000, SDS-PAGE
dimer
-
2 * 37000, SDS-PAGE
dimer
Arthrobacter ureafaciens a62-1
-
2 * 37000, SDS-PAGE
-
dimer
Arthrobacter ureafaciens A51-1
-
2 * 38000, SDS-PAGE
-
monomer
-
1 * 46000, gel filtration
monomer
-
1 * 40000, gel filtration
monomer
Arthrobacter MC12493
-
1 * 40000, gel filtration
-
monomer
Arthrobacter globiformis S14-3
-
1 * 46000, gel filtration
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3
-
stable at 25C
644467
4
-
30C, 30 min., stable
644466
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
stable, rapid inactivation above 80C
644467
70
-
pH 6, 30 min., 80% activity
644466
75
-
pH 5.5, 1 h, stable up to
664404
75
-
pH 5.5, 20 min, stable up to
664513
80
-
30 min., complete activity loss
644466
80
-
pH 5.5, 1 h, about 20% loss of activity
664404
80
-
pH 5.5, 20 min, complete inactivation
664513
85
-
pH 5.5, 1 h, complete inactivation
664404
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli MV1184
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nutrition
-
di-D-fructofuranose-1,2':2,1'-dianhydride is expected to be useful as a low-calorie sweetener
nutrition
Arthrobacter MC12493
-
di-D-fructofuranose-1,2':2,1'-dianhydride is expected to be useful as a low-calorie sweetener
-