Information on EC 4.2.2.17 - inulin fructotransferase (DFA-I-forming)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Arthrobacter

EC NUMBER
COMMENTARY
4.2.2.17
-
RECOMMENDED NAME
GeneOntology No.
inulin fructotransferase (DFA-I-forming)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride (DFA I) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-fructosyl-D-fructofuranosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2->1)-beta-D-fructan lyase (alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,1'-dianhydride-forming)
This enzyme, like EC 4.2.2.16 [levan fructotransferase (DFA-IV-forming)] and EC 4.2.2.18 [inulin fructotransferase (DFA-III-forming)] eliminates the fructan chain from the terminal disaccharide leaving a difructose dianhydride. These enzymes have long been known as fructotransferases, so this is retained in the accepted name. Since the transfer is intramolecular, the reaction is an elimination and, hence, the enzyme is a lyase, belonging in EC 4.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
EC 2.4.1.200
-
-
formerly
-
inulin D-fructosyl-D-fructosyltransferase (1,2':2',1-dianhydride-forming)
-
-
-
-
inulin fructotransferase (depolymerizing, difructofuranose-1,2':2',1-dianhydride-forming)
-
-
-
-
inulin fructotransferase (DFA I-producing)
-
-
inulin fructotransferase (DFA I-producing)
Arthrobacter ureafaciens a62-1
-
-
-
inulin fructotransferase (DFA-I-producing)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
125008-19-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
MC12493
-
-
Manually annotated by BRENDA team
Arthrobacter globiformis S14-3
S14-3
-
-
Manually annotated by BRENDA team
Arthrobacter MC12493
MC12493
-
-
Manually annotated by BRENDA team
strain A51-1
-
-
Manually annotated by BRENDA team
strain a62-1
-
-
Manually annotated by BRENDA team
Arthrobacter ureafaciens A51-1
strain A51-1
-
-
Manually annotated by BRENDA team
Arthrobacter ureafaciens a62-1
strain a62-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter, Arthrobacter MC12493
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter ureafaciens a62-1
-
-
-
-
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter ureafaciens A51-1
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter, Arthrobacter MC12493
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + H2O
show the reaction diagram
Arthrobacter globiformis S14-3
-
-
i.e. DFA I
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Fe3+
-
1 mM, strong
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
slight stimulation
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4
-
inulin
-
pH 5.5, 45C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
8
-
10% of maximal activity at pH 3.5, 25% of maximal activity at pH 8.0
4.5
7
-
pH 4.5: about 50% of maximal activity, pH 7.0: about 50% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
60
-
30C: about 70% of maximal activity, 60C: about 45% of maximal activity
30
65
-
30C: about 80% of maximal activity
30
-
-
80% of maximal activity at 30C, 20% of maximal activity at 65C
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Arthrobacter ureafaciens a62-1
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Arthrobacter globiformis S14-3
-
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
39000
-
-
SDS-PAGE
40000
-
-
SDS-PAGE and gel filtration
41600
-
-
amino acid analysis
46000
-
-
gel filtration
60000
-
-
gel filtration
61000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 38000, SDS-PAGE
dimer
-
2 * 37000, SDS-PAGE
dimer
Arthrobacter ureafaciens A51-1
-
2 * 38000, SDS-PAGE
-
dimer
Arthrobacter ureafaciens a62-1
-
2 * 37000, SDS-PAGE
-
monomer
-
1 * 40000, gel filtration
monomer
-
1 * 46000, gel filtration
monomer
Arthrobacter globiformis S14-3
-
1 * 46000, gel filtration
-
monomer
Arthrobacter MC12493
-
1 * 40000, gel filtration
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
-
-
stable at 25C
4
-
-
30C, 30 min., stable
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
stable, rapid inactivation above 80C
70
-
-
pH 6, 30 min., 80% activity
75
-
-
pH 5.5, 1 h, stable up to
75
-
-
pH 5.5, 20 min, stable up to
80
-
-
30 min., complete activity loss
80
-
-
pH 5.5, 1 h, about 20% loss of activity
80
-
-
pH 5.5, 20 min, complete inactivation
85
-
-
pH 5.5, 1 h, complete inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli MV1184
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
nutrition
-
di-D-fructofuranose-1,2':2,1'-dianhydride is expected to be useful as a low-calorie sweetener
nutrition
Arthrobacter MC12493
-
di-D-fructofuranose-1,2':2,1'-dianhydride is expected to be useful as a low-calorie sweetener
-