4.2.1.163: 2-oxo-hept-4-ene-1,7-dioate hydratase

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For detailed information about 2-oxo-hept-4-ene-1,7-dioate hydratase, go to the full flat file.

Reaction

Synonyms

2-oxohept-3-ene-l,7-dioic acid hydratase, HpcG, OHED hydratase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.163 2-oxo-hept-4-ene-1,7-dioate hydratase

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Results	in table
112	AA Sequence
2	Cloned(Commentary)
2	Crystallization (Commentary)
3	Metals/Ions
1	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
15	Organism
1	Pathway
2	Purification (Commentary)
1	Reaction
7	Reference
10	Substrates and Products (Substrate)
3	Subunits
13	Synonyms
1	Systematic Name

Crystallization

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Crystallization on EC 4.2.1.163 - 2-oxo-hept-4-ene-1,7-dioate hydratase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

hanging drop vapor diffusion method, crystals of the native and selenomethionine-labelled enzyme are grown at 0.1M MES (pH 6.0), 0.7 M potassium thiocyanate, 7% (w/v) PEG 12000, 5% (w/v) PEG 550 MME, 10 mM dithiothreitol and 0.1 M Tris (pH 7.0), 0.7 M potassium thiocyanate, 6.4% (w/v) PEG 20000, 7% (w/v) PEG 550 MME,10 mM dithiothreitol, respectively

Escherichia coli

Q46982

736631

sitting drop vapor diffusion method, using 6.4% (w/v) polyethylene glycol 20000, 5% (w/v) polyethylene glycol 550 monomethyl ether, 0.7 M potassium thiocyanate, in 50 mM MES pH 5.5

Escherichia coli

P42270

735376