Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) and B834(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystals of the native and selenomethionine-labelled enzyme are grown at 0.1M MES (pH 6.0), 0.7 M potassium thiocyanate, 7% (w/v) PEG 12000, 5% (w/v) PEG 550 MME, 10 mM dithiothreitol and 0.1 M Tris (pH 7.0), 0.7 M potassium thiocyanate, 6.4% (w/v) PEG 20000, 7% (w/v) PEG 550 MME,10 mM dithiothreitol, respectively | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity. The enzyme shows a slight preference for Mn2+ over Mg2+ | Escherichia coli | |
Mn2+ | required for activity. The enzyme shows a slight preference for Mn2+ over Mg2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4Z)-2-oxohept-4-enedioate + H2O | Escherichia coli | - |
(4S)-4-hydroxy-2-oxoheptanedioate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q46982 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4Z)-2-oxohept-4-enedioate + H2O | - |
Escherichia coli | (4S)-4-hydroxy-2-oxoheptanedioate | - |
r | |
additional information | the enzyme cannot process 2-oxo-3-heptene-1,7 dioate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homopentamer | 2 * 000, calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
HpcG | - |
Escherichia coli |