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4.2.1.163: 2-oxo-hept-4-ene-1,7-dioate hydratase

This is an abbreviated version!
For detailed information about 2-oxo-hept-4-ene-1,7-dioate hydratase, go to the full flat file.

Reaction

(4Z)-2-oxohept-4-enedioate
+
H2O
=
(4S)-4-hydroxy-2-oxoheptanedioate

Synonyms

2-oxohept-3-ene-l,7-dioic acid hydratase, HpcG, OHED hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.163 2-oxo-hept-4-ene-1,7-dioate hydratase

Purification

Purification on EC 4.2.1.163 - 2-oxo-hept-4-ene-1,7-dioate hydratase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, HisTrap column chromatography, and Superdex 75 gel filtration
Q Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration