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4.2.1.122: tryptophan synthase (indole-salvaging)

This is an abbreviated version!
For detailed information about tryptophan synthase (indole-salvaging), go to the full flat file.

Reaction

L-serine
+
indole
=
L-tryptophan
+
H2O

Synonyms

PfTrpB, PfTrpS, StTrpS (beta2), Trp synthase, TrpB2, TrpBbeta, TRPS, tryptophan synthase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.122 tryptophan synthase (indole-salvaging)

Cloned

Cloned on EC 4.2.1.122 - tryptophan synthase (indole-salvaging)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
-
gene trpB1, phylogenetic tree, expression in Escherichia coli strain BL21, transcript levels of trpB1 in the host, DELTAtrpB1 strains by quantitative RT-PCR expression analysis
gene trpB2, phylogenetic tree, expression in Escherichia coli strain BL21, transcript levels of trpB2 in the host, DELTAtrpB2 strains by quantitative RT-PCR expression analysis
gene trpS, recombinant expression in Escherichia coli
-
recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
recombinant expression of N-terminally His6-tagged TrpB in Escherichia coli strain KRX