4.2.1.122: tryptophan synthase (indole-salvaging)

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For detailed information about tryptophan synthase (indole-salvaging), go to the full flat file.

Reaction

Synonyms

PfTrpB, PfTrpS, StTrpS (beta2), Trp synthase, TrpB2, TrpBbeta, TRPS, tryptophan synthase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.122 tryptophan synthase (indole-salvaging)

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Results	in table
30	AA Sequence
1	Application
7	Cloned(Commentary)
1	Cofactor
2	Expression
11	General Information
13	kcat/KM [mM/s]
15	KM Value [mM]
5	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
9	Organism
1	Pathway
3	pH Optimum
1	pI Value
13	Protein Variants
3	Purification (Commentary)
3	Reaction
9	Reference
37	Substrates and Products (Substrate)
6	Subunits
11	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
12	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 4.2.1.122 - tryptophan synthase (indole-salvaging)

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

L-serine + indole

L-tryptophan + H2O

6 entries

L-threonine + indole

(2S,3S)-beta-methyltryptophan + H2O

2 entries

additional information

?

-

Pyrococcus furiosus

-

the rate of Thr deamination by PfTrpS is 8.5fold faster than with Ser, competitive with the rate of beta-substitution. Substrate differentiation mechanism of the enzyme, molecular dynamics simulations analysis, overview

747116

-

-

?

L-serine + indole

L-tryptophan + H2O

Psilocybe cubensis

A0A2U8JPY3

-

747575

-

-

?

L-serine + indole

L-tryptophan + H2O

Pyrococcus furiosus

-

-

748036

-

-

?

L-serine + indole

L-tryptophan + H2O

Pyrococcus furiosus

-

Ser and IGP react with a coupling efficiency of over 99% and only trace indole is released into solution by cells

747116

-

-

?

L-serine + indole

L-tryptophan + H2O

Salmonella enterica subsp. enterica serovar Typhimurium

-

L-serine is the preferred substrate

747549

-

-

?

L-serine + indole

L-tryptophan + H2O

Thermococcus kodakarensis

Q5JDJ1, Q9YGB0

-

727489

-

-

?

L-serine + indole

L-tryptophan + H2O

Thermococcus kodakarensis

-

the enzyme is involved in L-tryptophan biosynthesis. TrpB2 does not interact with TrpA as in the case of TrpB1. TrpB2 provides an alternate route to generate Trp from serine and free indole (indole salvage)

747735

-

-

?

L-threonine + indole

(2S,3S)-beta-methyltryptophan + H2O

Pyrococcus furiosus

-

-

748036

-

-

?

L-threonine + indole

(2S,3S)-beta-methyltryptophan + H2O

Pyrococcus furiosus

-

only 17% of the indole that is released from the alpha-subunit goes on to form beta-MeTrp, demonstrating that the release of indole is decoupled from product formation. Thr binds non-covalently to the isolated beta-subunit, indicating that the beta-methyl group hinders entry into the catalytic cycle. The beta-methyl of Thr causes a steric clash that destabilizes the E(Aex1) intermediate

747116

-

-

?