4.2.1.115: UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting)

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For detailed information about UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting), go to the full flat file.

Reaction

Synonyms

4,6-dehydratase/5-epimerase, CapE, Cj1293, FlaA1, HP0840, inverting 4,6-dehydratase, Mg534, More, NAD(P)+-dependent dehydratase/epimerase, PseB, UDP-alpha-D-GlcNAc modifying dehydratase, UDP-GlcNAc 5-inverting 4,6-dehydratase, UDP-GlcNAc C6 dehydratase, UDP-GlcNAc C6 dehydratase/C4 reductase, UDP-N-acetylglucosamine 5-inverting 4,6-dehydratase, WbpM

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.115 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting)

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Results	in table
2014	AA Sequence
1	Application
1	CAS Registry Number
6	Cloned(Commentary)
10	Cofactor
3	Crystallization (Commentary)
7	General Information
1	Inhibitors
6	KM Value [mM]
7	Localization
5	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
21	Organism
8	Pathway
4	PDB ID
10	pH Optimum
1	pI Value
26	Protein Variants
4	Purification (Commentary)
6	Reaction
17	Reference
4	Specific Activity [micromol/min/mg]
37	Substrates and Products (Substrate)
5	Subunits
36	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 4.2.1.115 - UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

recombinant GST-tagged enzyme from Escherichia coli strain BL-21 GOLD by glutathione affinity chromatography, proteolytic removal of the GST-tag, and ultrafiltration, recombinant His6-tagged enzyme from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography, proteolytic removal of the His-tag, another step of nickel affinity chromatography, and desalting gel filtration, thereby the recombinant protein corresponds to the native enzyme in which the initial methionine is replaced by GPGS sequence resulting from the cleavage site

Megavirus chiliensis

G5CSR9

748176

recombinant His-tagged FlaA1 9.9fold from Escherichia coli strain BL21(DE3) to homogeneity by nickel chelation and cation exchange chromatography

Helicobacter pylori

-

692952

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)

Campylobacter jejuni

-

691202

recombinant N-terminally His-tagged and C-terminally His-tagged WbpMs from Escherichia coli by selective solubilization from the inner membrane by lauryl sarcosine and nickel chelation chromatography

Pseudomonas aeruginosa

P72145

694211