4.2.1.115: UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting)

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For detailed information about UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting), go to the full flat file.

Reaction

Synonyms

4,6-dehydratase/5-epimerase, CapE, Cj1293, FlaA1, HP0840, inverting 4,6-dehydratase, Mg534, More, NAD(P)+-dependent dehydratase/epimerase, PseB, UDP-alpha-D-GlcNAc modifying dehydratase, UDP-GlcNAc 5-inverting 4,6-dehydratase, UDP-GlcNAc C6 dehydratase, UDP-GlcNAc C6 dehydratase/C4 reductase, UDP-N-acetylglucosamine 5-inverting 4,6-dehydratase, WbpM

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.115 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting)

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Results	in table
2014	AA Sequence
1	Application
1	CAS Registry Number
6	Cloned(Commentary)
10	Cofactor
3	Crystallization (Commentary)
7	General Information
1	Inhibitors
6	KM Value [mM]
7	Localization
5	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
21	Organism
8	Pathway
4	PDB ID
10	pH Optimum
1	pI Value
26	Protein Variants
4	Purification (Commentary)
6	Reaction
17	Reference
4	Specific Activity [micromol/min/mg]
37	Substrates and Products (Substrate)
5	Subunits
36	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Turnover Number [1/s]

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Cloned on EC 4.2.1.115 - UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting)

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

gene flaA1, DNA and amino acid sequence determination and analysis

Brachyspira hyodysenteriae

-

692816

gene flaA1, DNA and amino acid sequence determination, expression analysis

Helicobacter pylori

Q6VYQ5, Q6VYQ6

692826

gene flaA1, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), complementation of a Pseudomonas aeruginosa WbpM knockout by expression of His-tagged FlaA1

Helicobacter pylori

-

692952

gene mg534, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL-21 GOLD, or recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta(DE3)

Megavirus chiliensis

G5CSR9

748176

gene pseB, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Campylobacter jejuni

-

691202

overexpression of wild-type N-terminally His-tagged and C-terminally His-tagged WbpMs, and mutant truncated WbpM in membranes of Escherichia coli, reaction yields are lower with C-terminally His-tagged than with N-terminally His-tagged recombinant enzyme

Pseudomonas aeruginosa

P72145

694211