4.1.2.45: trans-o-hydroxybenzylidenepyruvate hydratase-aldolase

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For detailed information about trans-o-hydroxybenzylidenepyruvate hydratase-aldolase, go to the full flat file.

Reaction

Synonyms

2'-hydroxybenzalpyruvate aldolase, fHBP HA A, fHBP HA B, nsaE, o-tHBPA hydratase-aldolase, tHBP-HA, tHBPA hydratase-aldolase, trans-o-hydroxybenzylidenepyruvate hydratase-aldolase

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.2 Aldehyde-lyases

                4.1.2.45 trans-o-hydroxybenzylidenepyruvate hydratase-aldolase

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Results	in table
20	AA Sequence
3	Cloned(Commentary)
1	Cofactor
1	General Stability
10	Inhibitors
1	Ki Value [mM]
8	KM Value [mM]
8	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
28	Organism
5	Pathway
2	PDB ID
3	pH Optimum
4	pH Range
3	pH Stability
3	pI Value
4	Purification (Commentary)
1	Reaction
2	Reaction Type
18	Reference
4	Specific Activity [micromol/min/mg]
76	Substrates and Products (Substrate)
9	Subunits
17	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]

Temperature Stability

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Temperature Stability on EC 4.1.2.45 - trans-o-hydroxybenzylidenepyruvate hydratase-aldolase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

30 - 40

Pseudomonas fluorescens

-

after 30 min incubation, the decreases of the activity are 64% at 40°C and 24% at 35°C, while at 30°C, the activity remains unchanged. However, at 30°C, the decreases of the activity are 24% after 5 h and 54% after 24 h

714354

50

Sphingomonas paucimobilis

-

pH 7.3, 20 mM Tris-HCl buffer, 5 min, enzyme form fHBP HA A and enzyme form fHBP HA B, stable up to

698644

65

Sphingomonas paucimobilis

-

pH 7.3, 20 mM Tris-HCl buffer, 5 min, enzyme form fHBP HA A and enzyme form fHBP HA B, 80% loss of activity

698644