3.8.1.8: atrazine chlorohydrolase
This is an abbreviated version!
For detailed information about atrazine chlorohydrolase, go to the full flat file.
Word Map on EC 3.8.1.8
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3.8.1.8
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herbicide
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adp
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dechlorination
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hydroxyatrazine
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arthrobacter
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s-triazine
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amidohydrolase
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nocardioides
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melamine
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atrazine-degrading
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bioremediation
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agriculture
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haematococcus
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ametryn
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cyanuric
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aurescens
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phytoremediation
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analysis
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environmental protection
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biotechnology
- 3.8.1.8
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herbicide
- adp
-
dechlorination
- hydroxyatrazine
- arthrobacter
- s-triazine
-
amidohydrolase
- nocardioides
- melamine
-
atrazine-degrading
-
bioremediation
- agriculture
- haematococcus
- ametryn
-
cyanuric
- aurescens
-
phytoremediation
- analysis
- environmental protection
- biotechnology
Reaction
Synonyms
atrazine chlorohydrolase, atrazine chlorohydrolase 2, atrazine dechlorinase, AtzA, AtzB, dechlorinase, atrazine (9CI), hydroxyatrazine N-ethylaminohydrolase, More, triazine hydrolase, TrZN
ECTree
3.8.1.8 atrazine chlorohydrolaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.8.1.8 - atrazine chlorohydrolase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.02
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine
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deamination
0.04
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine
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deamination
3.9
ametryn
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wild-type enzyme, pH 7.0, temperature not specified in the publication
7.8
ametryn
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mutant R325, pH and temperature not specified in the publicationS
0.049
Atrazine
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A216A/T217D/T219E/A220A/D250D, site-directed mutagenesis, AtzA-variant 288, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.049
Atrazine
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T217D/T219E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.062
Atrazine
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A216G/T217D/T219G/A220H/D250D, site-directed mutagenesis, AtzA-variant 734, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.062
Atrazine
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A216G/T217D/T219G/A220H/D250D, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.067
Atrazine
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A216S/T217A/T219P/A220F/D250G, site-directed mutagenesis, AtzA-variant 357, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.067
Atrazine
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A216S/T217A/T219P/A220F/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.076
Atrazine
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A216A/T217S/T219H/A220G/D250Y, site-directed mutagenesis, AtzA-variant 431, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.076
Atrazine
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T217S/T219H/A220G/D250Y, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.09
Atrazine
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A216G/T217D/T219G/A220H/D250G, site-directed mutagenesis, AtzA-variant 430, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.092
Atrazine
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A216S/T217D/T219V/A220H/D250G, site-directed mutagenesis, AtzA-variant 297, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.092
Atrazine
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A216Y/T217D/T219Y/A220H/D250V, site-directed mutagenesis, AtzA-variant 422, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.092
Atrazine
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A216S/T217D/T219V/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.092
Atrazine
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A216Y/T217D/T219Y/A220H/D250V, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.093
Atrazine
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A216Y/T217D/T219T/A220H/D250E, site-directed mutagenesis, AtzA best variant consensus, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.093
Atrazine
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A216Y/T217D/A220H/D250E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.095
Atrazine
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A216G/T217D/T219A/A220V/D250W, site-directed mutagenesis, AtzA-variant 305, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.095
Atrazine
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A216G/T217D/T219A/A220V/D250W, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.1
Atrazine
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A216H/T217A/T219E/A220S/D250S, site-directed mutagenesis, AtzA-variant 662, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.1
Atrazine
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A216H/T217A/T219E/A220S/D250S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.105
Atrazine
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A216S/T217D/T219G/A220S/D250D, site-directed mutagenesis, AtzA-variant 841, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.105
Atrazine
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A216S/T217D/T219G/A220S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.131
Atrazine
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Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C
0.132
Atrazine
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V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C
0.138
Atrazine
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D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C
0.14
Atrazine
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V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C
0.142
Atrazine
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V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C
0.145
Atrazine
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V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C
0.153
Atrazine
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more than 0.153 mM. AtzA wild type, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.153
Atrazine
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AtzA wild-type, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.9
Atrazine
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A216G/T217D/T219G/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
3.1
Atrazine
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mutant E241Q, pH and temperature not specified in the publication
13.3
Atrazine
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mutant R325S, pH and temperature not specified in the publication
19
Atrazine
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wild-type enzyme, pH 7.0, temperature not specified in the publication
additional information
additional information
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reduction in Km observed in the AtzA variants has allowed the full kinetic profile for the AtzA-catalyzed dechlorination of atrazine to be determined for the first time, revealing the hitherto-unreported substrate cooperativity in AtzA
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additional information
additional information
kinetic parameters of diverse mutants relative to the wild-type values, overview
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