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3.8.1.8: atrazine chlorohydrolase

This is an abbreviated version!
For detailed information about atrazine chlorohydrolase, go to the full flat file.

Word Map on EC 3.8.1.8

Reaction

Atrazine
+
H2O
=
hydroxyatrazine
 +
chloride

Synonyms

atrazine chlorohydrolase, atrazine chlorohydrolase 2, atrazine dechlorinase, AtzA, AtzB, dechlorinase, atrazine (9CI), hydroxyatrazine N-ethylaminohydrolase, More, triazine hydrolase, TrZN

ECTree

     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.8 atrazine chlorohydrolase

Specific Activity

Specific Activity on EC 3.8.1.8 - atrazine chlorohydrolase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0001
Clavibacter michiganese
-
crude cell extract
0.0017
-
crude cell extract
0.0039
-
crude cell extract
0.0045
-
crude cell extract
0.0048
-
crude cell extract
0.0074
-
crude cell extract
0.0162
-
partially purified enzyme
0.16
-
crude extract
0.33
-
prurification step ammonium sulfate precipitation
1.05
-
prurification step ceramic hydroxyapatite type I column
10.3
-
substrate: atrazine, V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C
10.6
-
substrate: atrazine, D30G/I393P/L395S/N429S/V466A, pH 7.2, 23°C
10.7
-
substrate: atrazine, Q7R/V12A/S307P/M337T/I393R/L395R/S438G/R462W, pH 7.2, 23°C
12.2
-
substrate: atrazine, G55S/I393W/L395R/M315I/E433K, pH 7.2, 23°C
12.8
-
substrate: atrazine, V12P/D30G/Q71R/H249Y/270V/R389C/I393P/L395P, pH 7.2, 23°C
15.6
-
substrate: atrazine, V12A/Q71A/M315I/M341V, pH 7.2, 23°C
2.6
purified recombinant wild-type enzyme, 0.046 mM atrazine, pH 7.2, 25°C
3
-
substrate: atrazine, wild-type enzyme, pH 7.2, 23°C
3.6
-
substrate: atrazine, D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C
3.7
-
about, purified recombinant enzyme
4.5
-
purified enzyme
6.8
-
substrate: atrazine, V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C
6.9
-
substrate: atrazine, Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C
7.1
-
substrate: atrazine, V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C
7.3
-
substrate: atrazine, V12F/D30G/V58A/T121A/I393S/L395H, pH 7.2, 23°C
7.4
-
substrate: atrazine, Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C
8.6
-
substrate: atrazine, D30G/Q71G/M315I/R389C/H399Q/N429S/V466A, pH 7.2, 23°C
8.7
-
substrate: atrazine, V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C
9.6
-
substrate: atrazine, D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C