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3.8.1.8: atrazine chlorohydrolase

This is an abbreviated version!
For detailed information about atrazine chlorohydrolase, go to the full flat file.

Word Map on EC 3.8.1.8

Reaction

Atrazine
+
H2O
=
hydroxyatrazine
+
chloride

Synonyms

atrazine chlorohydrolase, atrazine chlorohydrolase 2, atrazine dechlorinase, AtzA, AtzB, dechlorinase, atrazine (9CI), hydroxyatrazine N-ethylaminohydrolase, More, triazine hydrolase, TrZN

ECTree

     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.8 atrazine chlorohydrolase

Purification

Purification on EC 3.8.1.8 - atrazine chlorohydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by ammonium sulfate precipitation and filtration using 100 and 50 kDa cut-off membrane filters
-
partially purified
-
recombinant from Escherichia coli
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, tag cleavage by by thrombin proteolysis, and again gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) solubilized from inclusion bodies by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
using a CHT ceramic hydroxyapatite type I column
-