3.6.4.B6: archaeal flagellar ATPase

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For detailed information about archaeal flagellar ATPase, go to the full flat file.

Reaction

Synonyms

ATPase FlaI, FkaI, flagellar accessory protein, FlaH, FlaI, motor ATPase, Saci_1173, SSO2316

ECTree

     3 Hydrolases

         3.6 Acting on acid anhydrides

             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement

                3.6.4.B6 archaeal flagellar ATPase

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Results	in table
1	Activating Compound
3	AA Sequence
7	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
2	Expression
30	General Information
1	Inhibitors
16	Localization
11	Metals/Ions
2	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
24	Organism
3	pH Optimum
1	pH Range
14	Protein Variants
3	Purification (Commentary)
3	Reaction
20	Reference
44	Substrates and Products (Substrate)
14	Subunits
37	Synonyms
3	Temperature Optimum [°C]
1	Temperature Range [°C]

Purification

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Purification on EC 3.6.4.B6 - archaeal flagellar ATPase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Sulfolobus acidocaldarius

Q4J9L0

724227

FlaI can be purified from the cytoplasm of Escherichia coli

Saccharolobus solfataricus

Q97WB7

725893

recombinant Strep II-tagged FlaI, Strep II-tagged FlaXc, and His6-tagged FlaH from in Escherichia coli strain BL21-CodonPlus-RIL as ternary complex by nickel affinity chromatography, or single FlaI by avidin affinity chromatography

Sulfolobus acidocaldarius

Q4J9L0

733831