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Literature summary for 3.6.4.B6 extracted from

  • Albers, S.V.; Driessen, A.J.
    Analysis of ATPases of putative secretion operons in the thermoacidophilic archaeon Sulfolobus solfataricus (2005), Microbiology, 151, 763-773.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane homologously expressed 6 * His-tagged FlaI in Sulfolobus localizes solely to membranes Saccharolobus solfataricus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Saccharolobus solfataricus
Mn2+ activates Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97WB7
-
-
Saccharolobus solfataricus P2 Q97WB7
-
-

Purification (Commentary)

Purification (Comment) Organism
FlaI can be purified from the cytoplasm of Escherichia coli Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Saccharolobus solfataricus ADP + phosphate
-
?
ATP + H2O
-
Saccharolobus solfataricus P2 ADP + phosphate
-
?
CTP + H2O about 30% of the activity with ATP Saccharolobus solfataricus CDP + phosphate
-
?
CTP + H2O about 30% of the activity with ATP Saccharolobus solfataricus P2 CDP + phosphate
-
?
GTP + H2O about 20% of the activity with ATP Saccharolobus solfataricus GDP + phosphate
-
?
GTP + H2O about 20% of the activity with ATP Saccharolobus solfataricus P2 GDP + phosphate
-
?
UTP + H2O about 50% of the activity with ATP Saccharolobus solfataricus UDP + phosphate
-
?
UTP + H2O about 50% of the activity with ATP Saccharolobus solfataricus P2 UDP + phosphate
-
?

Synonyms

Synonyms Comment Organism
FkaI
-
Saccharolobus solfataricus
SSO2316 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Saccharolobus solfataricus

Expression

Organism Comment Expression
Saccharolobus solfataricus FlaI mRNAS can be found only in stationary growth phase of arabinose- and maltose-grown cells. NO expression in media containing glucose or tryptone as sole carbon source up